The purpose of this study is to determine the effects of pudendal nerve stimulation (PNS) on reflex bladder activity and develop an animal model of underactive bladder (UAB). In six anesthetized cats, a bladder catheter was inserted via the urethra to infuse saline and measure pressure. A cuff electrode was implanted on the pudendal nerve. After determining the threshold intensity (T) for PNS to induce an anal twitch, PNS (5 Hz, 0.2 ms, 2T or 4T) was applied during cystometrograms (CMGs). PNS (4-6T) of 30-min duration was then applied repeatedly until bladder underactivity was produced. Following stimulation control CMGs were performed over 1.5-2 hours to determine the duration of bladder underactivity. When applied during CMGs, PNS (2T and 4T) significantly (p<0.05) increased bladder capacity while PNS at 4T also significantly (p<0.05) reduced bladder contraction amplitude, duration, and area under contraction curve. Repeated application of 30-min PNS for a cumulative period of 3-8 hours produced bladder underactivity exhibiting a significantly (p<0.05) increased bladder capacity (173±14% of control) and a significantly (p<0.05) reduced contraction amplitude (50±7% of control). The bladder underactivity lasted more than 1.5-2 hours after termination of the prolonged PNS. These results provide basic science evidence supporting the proposal that abnormal afferent activity from external urethral/anal sphincter could produce central inhibition that underlies non-obstructive urinary retention (NOUR) in Fowler's syndrome. This cat model of UAB may be useful to investigate the mechanism by which sacral neuromodulation reverses NOUR in Fowler's syndrome.

中文翻译：

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猫阴部传入活动延长引起的膀胱活动不足


本研究的目的是确定阴部神经刺激（PNS）对膀胱反射活动的影响，并开发膀胱活动不足（UAB）动物模型。在六只麻醉猫中，通过尿道插入膀胱导管以注入盐水并测量压力。将袖带电极植入阴部神经。确定 PNS 诱发肛门抽搐的阈值强度 (T) 后，在膀胱内压描记图 (CMG) 期间应用 PNS（5 Hz、0.2 ms、2T 或 4T）。然后重复应用 30 分钟持续时间的 PNS (4-6T)，直到产生膀胱活动不足。刺激控制 CMG 进行 1.5-2 小时，以确定膀胱活动不足的持续时间。在 CMG 期间应用时，PNS（2T 和 4T）显着（p<0.05）增加膀胱容量，而 4T 时的 PNS 也显着（p<0.05）减少膀胱收缩幅度、持续时间和收缩曲线下面积。重复应用 30 分钟 PNS，累积时间为 3-8 小时，导致膀胱活动不足，表现出显着 (p<0.05) 增加的膀胱容量（对照的 173±14%）和显着 (p<0.05) 降低的收缩幅度（对照的 50±7%）。长期 PNS 终止后，膀胱活动不足持续超过 1.5-2 小时。这些结果提供了基础科学证据，支持以下观点：尿道外括约肌/肛门括约肌的异常传入活动可能产生中枢抑制，而中枢抑制是福勒综合征中非梗阻性尿潴留 (NOUR) 的基础。这种 UAB 猫模型可能有助于研究福勒综合征中骶神经调节逆转 NOUR 的机制。