For successful batch farrowing, porcine oestrus and ovulation must be synchronized using fixed‐time artificial insemination (FTAI). However, exogenous gonadotropins, which are currently used in FTAI, negatively affect gilt ovulation. Here, we aimed to improve sexually mature gilt superovulation efficiency using passive immunization against inhibin during FTAI. Altrenogest‐treated gilts were challenged with 10 ml anti‐inhibin serum (AIS group, n = 6), 1,000 IU pregnant mare serum gonadotropin (PMSG group, n = 6), or 10 ml goat serum (control group, n = 6). Gilts in the AIS and PMSG groups were inseminated according to the FTAI protocol, and gilts in the control group were inseminated during natural oestrus. When PMSG was replaced by AIS during FTAI of gilts, ovulation rate and embryos recovered were significantly greater in the AIS group as compared to the other two groups (p < .05). Especially the average number of 6–8‐cell embryos in the AIS group was significantly higher than that in the PMSG group (p < .01). Moreover, the blastocyst number in the AIS group was significantly higher than that in the PMSG group and the control group (p < .05). But there was no significant difference in the blastocyst number between the PMSG group and the control group (p > .05). Besides, plasma levels of estradiol‐β (E2) and progesterone (P4) were significantly greater in the AIS group as compared to the other two groups on Day 23 and D 27, respectively (p < .01). In summary, we devised an improved high‐yield FTAI protocol for sexually mature gilts using AIS; this protocol had a greater superovulation efficiency than the FTAI using PMSG.

中文翻译：

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抗抑制素血清疗法与定时人工授精相结合改善后备母猪排卵

为了成功分批分娩，必须使用固定时间的人工授精（FTAI）同步猪的发情期和排卵期。但是，目前在FTAI中使用的外源性促性腺激素对后备排卵有负面影响。在这里，我们旨在通过在FTAI期间使用抑制素的被动免疫来提高性成熟的后备母猪超排卵效率。用10 ml抗抑制素血清（AIS组，n  = 6），1,000 IU怀孕母马血清促性腺激素（PMSG组，n  = 6）或10 ml山羊血清（对照组，n = 6）。根据FTAI规程对AIS和PMSG组的后备母猪进行授精，在自然发情期对对照组的后备母猪进行授精。在小母猪FTAI期间用AIS代替PMSG时，与其他两组相比，AIS组的排卵率和恢复的胚胎显着增加（p  <.05）。尤其是，AIS组的6-8细胞胚胎的平均数量显着高于PMSG组（p  <.01）。此外，AIS组的胚泡数显着高于PMSG组和对照组（p  <.05）。但PMSG组与对照组的囊胚数无显着差异（p > .05）。此外，AIS组的血浆雌二醇-β（E2）和孕酮（P4）水平分别在第23天和D 27时显着高于其他两组（p  <.01）。总而言之，我们为使用AIS进行性成熟的后备母猪设计了一种改良的高产FTAI方案。该协议比使用PMSG的FTAI具有更高的超排卵效率。