Label‐free optical antibody testing kit based on a self‐assembled whispering‐gallery‐mode microsphere,Journal of Biophotonics

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Label‐free optical antibody testing kit based on a self‐assembled whispering‐gallery‐mode microsphere
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2020-11-05 , DOI: 10.1002/jbio.202000338
Ying Yue ₁ , Hui Ding ₁ , Chen Chen ₁

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The appearance of antibodies in blood is a critical signal to suggest the infection. A rapid and accurate detection method for the antibody is significant to the disease diagnosis, especially for the epidemic. To this end, a highly sensitive whispering‐gallery‐mode (WGM) optical testing kit is designed and fabricated for detecting the specific immunoglobulin antibodies. The key component of the kit is a silica self‐assembled microsphere decorated with the nucleocapsid proteins (N‐proteins) of the SARS‐CoV‐2 virus. After the N‐protein antibody immunoglobulin G (N‐IgG) and immunoglobulin M (N‐IgM) solutions being injected into the kit, the WGM red‐shifts due to the antigen–antibody reaction. The wavelength displacement rates are proportional to the concentrations of these two antibodies from 1 to 100 μg/mL. A good specificity of the kit is demonstrated by the nonspecific human immunoglobulin G (H‐IgG) and immunoglobulin M (H‐IgM).

中文翻译：

基于自组装耳语画廊模式微球的无标签光学抗体检测试剂盒

血液中抗体的出现是表明感染的关键信号。快速，准确的抗体检测方法对疾病诊断，尤其是对流行病具有重要意义。为此，设计并制造了一种高度灵敏的耳语图库模式（WGM）光学检测试剂盒，用于检测特定的免疫球蛋白抗体。该试剂盒的关键组件是二氧化硅自组装微球，上面装饰着SARS-CoV-2病毒的核衣壳蛋白（N蛋白）。将N蛋白抗体免疫球蛋白G（N‐IgG）和免疫球蛋白M（N‐IgM）溶液注入试剂盒后，由于抗原抗体反应，WGM发生红移。波长位移率与这两种抗体的浓度成正比，范围为1至100μg/ mL。

更新日期：2020-11-05

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