Aeromonas hydrophila is ubiquitous in the aquaculture industry and a constant cause of severe disease and economic losses. The early diagnosis of these infections is crucial for disease surveillance and prevention. We developed a real‐time recombinase polymerase amplification (real‐time RPA) assay for detection of A. hydrophila using the haemolysin gene. The assay was performed at 37°C for 20 min and was highly specific with no cross‐reaction with other fish pathogens or with other Aeromonas species. The assay detection limit was 10² copies of the Aeromonas hydrophila per reaction. Compared with traditional culture‐based method or real‐time PCR, the diagnostic sensitivity and specificity of the real‐time RPA were 73.7 and 100%, as well as 64.7 and 93%. Our newly developed real‐time RPA was specific and sensitive and can be used in large‐scale and point‐of‐care field investigations of A. hydrophila infections to enable earlier diagnoses.

中文翻译：

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开发用于实时检测嗜水气单胞菌的实时重组酶聚合酶扩增法

嗜水气单胞菌在水产养殖业中无处不在，并且是造成严重疾病和经济损失的持续原因。这些感染的早期诊断对于疾病的监测和预防至关重要。我们开发了一种实时重组酶聚合酶扩增（实时RPA）测定法，用于使用溶血素基因检测亲水性链球菌。该测定法在37°C下进行20分钟，具有高度特异性，与其他鱼类病原体或其他气单胞菌物种无交叉反应。该方法的检出限为10 ²份嗜水气单胞菌每个反应。与传统的基于文化的方法或实时PCR相比，实时RPA的诊断敏感性和特异性分别为73.7和100％，以及64.7和93％。我们新开发的实时RPA具有特异性和敏感性，可用于亲水性链球菌感染的大规模和现场即时调查，以实现早期诊断。