Abstract A simple method for immobilization of amylase (Am) on montmorillonite (Mt) was developed. Direct immobilization of crude commercial Am was initially evaluated by size exclusion chromatography by considering Am peaks (in comparison to the pure enzyme from the same source), while the immobilized enzyme performance was monitored by anion exchange chromatography. The immobilized Am showed approximately 55.6 ± 0.7% (n = 3) of the activity of the free enzyme. Both free and immobilized Am showed maximum performance in neutral pH (pH ∼ 7). The immobilized Am maintained more than 60% of its initial activity after 10 reuse cycles. The immobilized enzyme was successfully applied for starch removal in crude carbohydrates extracted from medicinal plant samples.

中文翻译：

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尺寸排阻色谱法评价淀粉酶在纳米粘土上的固定化及其在草药植物提取物中淀粉去除中的应用

摘要 开发了一种将淀粉酶 (Am) 固定在蒙脱石 (Mt) 上的简单方法。粗品商业 Am 的直接固定化最初是通过尺寸排阻色谱法通过考虑 Am 峰（与来自相同来源的纯酶相比）进行评估的，而固定化酶的性能则通过阴离子交换色谱法进行监测。固定化的 Am 显示出大约 55.6 ± 0.7% (n = 3) 的游离酶活性。游离的和固定的 Am 在中性 pH 值（pH 〜 7）中都表现出最大的性能。在 10 次重复使用循环后，固定化的 Am 保持了其初始活性的 60% 以上。该固定化酶已成功应用于从药用植物样品中提取的粗碳水化合物中的淀粉去除。