The pathogenic yeast strain LIAO causing the milky disease in the Chinese mitten crab belonged to one member of Metschnikowia bicuspidate which could grow well at different temperatures from 28 to 4 °C. It was also found that the pathogenic yeast strain LIAO could grow in the extracts of the muscle, gill, heart tissues, intestinal tracts of the healthy Chinese mitten crabs by using the reducing sugars, amino acids and other nutrients in them. Massoia lactone released from liamocins produced by Aureobasidium melanogenum had high anti-fungal activity against the pathogenic yeast strain LIAO and M. bicuspidate WCY isolated from the diseased marine crabs. The minimal inhibitory concentrations (MIC) and the minimal fungicidal concentration (MFC) in the liquid culture against the pathogenic yeast strain LIAO were 0.15 mg/mL and 0.34 mg/mL, respectively. Massoia lactone as a bio-surfactant could damage the cell membrane, even break the whole cells of the pathogenic yeast strain LIAO and cause cellular necrosis of the pathogenic yeast LIAO. Therefore, Massoia lactone could be used to effectively kill the pathogenic yeast strains and as an effectitve treatment for milky disease in the Chinese mitten crab.

在中华绒螯蟹中引起乳状病的致病性酵母菌株LIAO属于双尖顶小球藻的一个成员，在28至4°C的不同温度下均可生长良好。还发现，致病性酵母菌株LIAO可以通过使用健康的中华绒螯蟹中的还原糖，氨基酸和其他营养物质在其肌肉，腮，心脏组织，肠道提取物中生长。从产黑色素单胞菌产生的藤黄素中释放出的马索亚内酯对致病性酵母菌株LIAO和二尖瓣分枝杆菌具有很高的抗真菌活性。从患病的海蟹中分离出的WCY。液体培养物中对致病性酵母菌株LIAO的最小抑制浓度（MIC）和最小杀真菌浓度（MFC）分别为0.15 mg / mL和0.34 mg / mL。Massoia内酯作为一种生物表面活性剂可能会破坏细胞膜，甚至破坏致病性酵母菌株LIAO的整个细胞，并导致致病性酵母LIAO的细胞坏死。因此，马尾藻内酯可以有效地杀死致病的酵母菌株，并有效地治疗中华绒螯蟹的乳状疾病。