Replenishing neurons in patients with neurodegenerative diseases is one of the ultimate therapies for these progressive, debilitating and fatal diseases. Electrical stimulation can improve neuron stem cell differentiation but requires a reliable nanopatterned electroconductive substrate. Potential candidate substrates are polycaprolactone (PCL) – polyaniline:camphorsulfonic acid (PANI:CSA) nanofibers, but their nanobiophysical properties need to be finetuned. The present study investigates the use of the pseudo-doping effect on the optimization of the electroconductivity of these polyaniline-based electrospun nanofibers. This was performed by developing a new solvent system that comprises a mixture of hexafluoropropanol (HFP) and trifluoroethanol (TFE). For the first time, an electroconductivity so high as 0.2 S cm^-1 was obtained for, obtained from a TFE:HFP 50/50 vol% solution, while maintaining fiber biocompatibility. The physicochemical mechanisms behind these changes were studied. The results suggest HFP promotes changes on PANI chains conformations through pseudo-doping, leading to the observed enhancement in electroconductivity. The consequences of such change in the nanofabrication of PCL-PANI fibers include an increase in fiber diameter (373 ± 172 nm), a decrease in contact angle (42 ± 3 °) and a decrease in Young Modulus (1.6 ± 0.5 MPa), making these fibers interesting candidates for neural tissue engineering. Electrical stimulation of differentiating neural stem cells was performed using AC electrical current. Positive effects on cell alignment and gene expression (DCX, MAP2) are observed. The novel optimized platform shows promising applications for (1) building in vitro platforms for drug screening, (2) interfaces for deep-brain electrodes; and (3) fully grown and functional neurons transplantation.

在神经退行性疾病患者中补充神经元是这些进行性，衰弱性和致命性疾病的最终疗法之一。电刺激可以改善神经元干细胞的分化，但需要可靠的纳米图案导电底物。潜在的潜在底物是聚己内酯（PCL）–聚苯胺：樟脑磺酸（PANI：CSA）纳米纤维，但需要对其纳米生物物理特性进行微调。本研究调查了伪掺杂效应在这些聚苯胺基电纺纳米纤维的电导率优化中的应用。这是通过开发一种新的溶剂系统完成的，该系统包含六氟丙醇（HFP）和三氟乙醇（TFE）的混合物。导电率首次高达0.2 S cm ^-1在保持纤维生物相容性的同时，从TFE：HFP 50/50 vol％溶液中获得了H.O。研究了这些变化背后的物理化学机理。结果表明，HFP通过伪掺杂促进PANI链构象的变化，从而导致观察到的导电性增强。PCL-PANI纤维纳米加工中这种变化的后果包括纤维直径增加（373±172 nm），接触角减小（42±3°）和杨氏模量减小（1.6±0.5 MPa），使这些纤维成为神经组织工程的有趣候选物。使用交流电流对分化的神经干细胞进行电刺激。观察到对细胞排列和基因表达（DCX，MAP2）的积极影响。用于药物筛选的体外平台，（2）深脑电极的接口；（3）完全生长且功能正常的神经元移植。