<br>山参通过L6肌管中叉头盒O3减少肌肉RING指蛋白-1和atrogin1抑制骨骼肌萎缩,Journal of Ethnopharmacology

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山参通过L6肌管中叉头盒O3减少肌肉RING指蛋白-1和atrogin1抑制骨骼肌萎缩
Journal of Ethnopharmacology ( IF 4.8 ) Pub Date : 2020-11-05 , DOI: 10.1016/j.jep.2020.113557
Young Mi Seok ₁ , Jae-Myung Yoo ₁ , Yoonju Nam ₁ , Jungeun Kim ₁ , Jin Soo Kim ₁ , Jun-Ho Son ₁ , Hyo Jung Kim ₁

Affiliation

民族药理学相关性

山参（ Panax ginseng CA Meyer）是一种具有免疫作用、肌肉损伤保护和能量代谢作用的药草。然而，山参在地塞米松（DEXA）通过叉头盒O（FOXO）家族引起的肌肉萎缩中的药理作用尚不清楚。因此，我们假设山参通过 L6 肌管中的 FOXO3 减少肌肉环指蛋白-1 (MuRF1) 和 atrogin1 来抑制骨骼肌萎缩。

方法

将大鼠成肌细胞 (L6) 细胞或 Sprague-Dawley (SD) 大鼠暴露于 DEXA 和山参。使用蛋白质印迹分析法分析肌肉萎缩靶标的表达，例如 MuRF1、atrogin1、MyHC（肌球蛋白重链）、HSP90、p-Akt、Akt、p-ERK1/2、ERK、FOXO3a、FOXO1、肌生长抑制素和卵泡抑素或实时PCR。测量肌管的直径。通过染色质免疫沉淀 (ChIP) 测定来分析糖皮质激素受体 (GR) 或 FOXO3a 的募集情况。

结果

山参治疗可减少 DEXA 诱导大鼠的肌肉重量损失和胶原蛋白沉积。山参治疗导致 MuRF1、atrogin1、p-ERK1/2、FOXO3a、FOXO1 和肌肉生长抑制素下降。此外，山参治疗导致肌管直径、MyHC、HSP90、p-Akt 和卵泡抑素的增加。山参处理降低了启动子上 GR、FOXO3a 和 RNA 聚合酶 II 的富集。

结论

这些结果表明，山参通过 L6 肌管中的 FOXO3a 减少 MuRF1 和 atrogin1 来抑制骨骼肌萎缩。

"点击查看英文标题和摘要"

Mountain ginseng inhibits skeletal muscle atrophy by decreasing muscle RING ﬁnger protein-1 and atrogin1 through forkhead box O3 in L6 myotubes

Ethnopharmacological relevance

Mountain ginseng (Panax ginseng C.A. Meyer) is a medicinal herb with immune effects, muscle damage protection and energy metabolism effects. However, the pharmacological role of mountain ginseng in dexamethasone (DEXA)-induced muscle atrophy through the forkhead box O (FOXO) family is not understood. Therefore, we hypothesized that mountain ginseng inhibits skeletal muscle atrophy by decreasing muscle RING ﬁnger protein-1 (MuRF1) and atrogin1 through FOXO3 in L6 myotubes.

Methods

Rat myoblast (L6) cells or Sprague-Dawley (SD) rats were exposed to DEXA and mountain ginseng. The expressions of muscle atrophy targets such as MuRF1, atrogin1, MyHC (myosin heavy chain), HSP90, p-Akt, Akt, p-ERK1/2, ERK, FOXO3a, FOXO1, myostatin, and follistatin were analyzed by using western blot analysis or real-time PCR. The diameter of myotubes was measured. Recruitment of glucocorticoid receptor (GR) or FOXO3a was analyzed by performing a chromatin immunoprecipitation (ChIP) assay.

Results

Mountain ginseng treatment reduced muscle weight loss and collagen deposition in DEXA-induced rats. Mountain ginseng treatment led to decreases in MuRF1, atrogin1, p-ERK1/2, FOXO3a, FOXO1, and myostatin. Also, mountain ginseng treatment led to increases in the diameter of myotubes, MyHC, HSP90, p-Akt, and follistatin. Treatment with mountain ginseng reduced enrichment of GR, FOXO3a, and RNA polymerase II on the promoters.