Agrimonia eupatoria L. is an important medicinal plant of the Rosaceae family. Antioxidant and volatile components have been identified in this herb. The seed dormancy of A. eupatoria causes time-consuming germination with a low percentage. In this study, the effects of treatments including 4 °C wet cold (24 h and 48 h), seed coat removal, potassium nitrate (0.2 %, 1%, and 3%), and gibberellic acid (100, 200, and 300 mg L⁻¹) were studied on seed dormancy breaking, seed germination indices, seedling growth parameters, the activity of α- amylase, antioxidant potential, as well as catalase and peroxidase activity in the in vitro conditions. The results showed that in coated seeds, seed coat removal was effective treatment on seed dormancy breaking, and increased the germination. The effect of 0.2 % potassium nitrate and 100 mg L⁻¹ gibberellic acid was very low, and 4 °C wet cold (24 h and 48 h) had no effect. The effect of treatments was more significant in uncoated seeds. The most effective treatments in uncoated seed were 0.2 % potassium nitrate and 100 mg L⁻¹ concentration of gibberellic acid, respectively. These treatments had a significant effect on germination indices, growth parameters, antioxidant potential, and α- amylase activity. Further, 1% potassium nitrate was the most suitable treatment for increasing the hypocotyl axis and seedling weight in uncoated seeds. In addition, 4 °C cold (24 h and 48 h) had a very little positive effect on all the mentioned indices. In all measurements, 3% potassium nitrate showed the weakest results.

Agrimonia eupatoria L.是蔷薇科的重要药用植物。该草药中已鉴定出抗氧化剂和挥发性成分。eupatoria的种子休眠导致费时的发芽率低。在这项研究中，处理的效果包括4°C湿冷（24 h和48 h），去除种皮，硝酸钾（0.2％，1％和3％）和赤霉素（100、200和300） mg L ^-1）在体外研究了种子休眠中断，种子发芽指数，幼苗生长参数，α-淀粉酶活性，抗氧化能力以及过氧化氢酶和过氧化物酶活性条件。结果表明，在包衣种子中，去除种皮是打破种子休眠的有效方法，并能提高发芽率。0.2％硝酸钾和100 mg L ^-1赤霉素的影响非常低，而4°C湿冷（24 h和48 h）则没有效果。在未包衣的种子中，处理效果更为显着。未包衣种子中最有效的处理方法是0.2％硝酸钾和100 mg L ^-1赤霉素的浓度分别。这些处理对发芽指数，生长参数，抗氧化剂潜力和α-淀粉酶活性具有重要影响。此外，1％硝酸钾是增加未包衣种子下胚轴和幼苗重量的最合适处理方法。此外，4°C的低温（24 h和48 h）对所有上述指标的影响很小。在所有测量中，3％的硝酸钾显示最弱的结果。