Herein, we isolated and purified polysaccharides from Craterellus cornucopioides. Chemical composition analysis revealed that both polysaccharides (CCPs-1 amd CCPs-2) were composed of different molar ratios of rhamnose, fucose, arabinose, xylose, mannose, glucose, and galactose. The structure of polysaccharides was determined using FT-IR, periodate oxidation, smith degradation, methylation analysis, and NMR, which demonstrated that the two polysaccharides were primarily connected by mannose with (1→3)-linked. Notably, the configuration of the two polysaccharides exhibited random coil with pyranoid polysaccharide containing α or β glycosidic bond. Furthermore, to increase the antioxidant activity of polysaccharides (CCPs-1 amd CCPs-2), two carboxymethylated polysaccharides were obtained as cmCCPs-1 and cmCCPs-2 with the degree of substitution values of 0.34 and 0.52, respectively. Structural analysis elucidated that nonselective carboxymethylation occurred in cmCCPs-1 and cmCCPs-2, where C-2, C-4 or C-6 were partially substituted. Of note, cmCCPs-2 exhibited the most significant antioxidant activity.

本文中，我们从山茱Cra中分离纯化了多糖。化学成分分析表明，两种多糖（CCPs-1和CCPs-2）均由鼠李糖，岩藻糖，阿拉伯糖，木糖，甘露糖，葡萄糖和半乳糖的不同摩尔比组成。使用FT-IR，高碘酸盐氧化，史密斯降解，甲基化分析和NMR确定了多糖的结​​构，这表明这两种多糖主要通过甘露糖（1→3）连接。值得注意的是，这两种多糖的构型与含有α或β的吡喃类多糖表现出无规卷曲糖苷键。此外，为了提高多糖（CCPs-1和CCPs-2）的抗氧化活性，获得了两种羧甲基化多糖，分别为cmCCPs-1和cmCCPs-2，取代度分别为0.34和0.52。结构分析表明，在cmCCPs-1和cmCCPs-2（其中C-2，C-4或C-6被部分取代）中发生了非选择性羧甲基化。值得注意的是，cmCCPs-2表现出最显着的抗氧化活性。