Peroxynitrite, one of the well-known reactive nitrogen species (RNS), which is considered to be an important oxidant. Besides, peroxynitrite plays an important role in the broad spectrum of biological processes, and it is also a vital participant in the emergence of cancer. To this end, the fluorescent probe PN-1 was rationally synthesized, which contains a coumarin-fused coumarin dye and borates as the specific recognition group. Probe PN-1 exhibits several obvious performances, for instance, high specificity, excellent sensitivity, and it could promptly respond toward ONOO⁻. Using this simple fluorescent probe, we achieved the imaging of endogenous and exogenous ONOO⁻ fluctuations in living cells and zebrafish. Moreover, PN-1 has efficiently differentiated cancerous cells from normal cells owing to image their different native ONOO⁻ levels, these results indicated that PN-1 is expected to be an analytical tool, for researching ONOO⁻ related cancer biology and studying the related effect of ONOO⁻ in food.

过氧亚硝酸盐，一种众所周知的活性氮物质（RNS），被认为是一种重要的氧化剂。此外，过氧亚硝酸盐在广泛的生物学过程中起着重要作用，并且也是癌症出现的重要参与者。为此，合理地合成了荧光探针PN-1，其包含香豆素融合的香豆素染料和硼酸盐作为特异性识别基团。探头PN-1表现出几个明显的表现，例如，特异性高，出色的灵敏度，并且它可以及时向ONOO响应^- 。使用这个简单的荧光探针，我们实现了内源性和外源性ONOO的成像^-在活细胞和斑马鱼的波动。此外，PN-1已经有效地分化癌细胞从由于图像他们本国的ONOO正常细胞^-的水平，这些结果表明，PN-1有望成为一个分析工具，对研究ONOO ^-有关的癌症生物学和研究ONOO的相关影响⁻食物中。