Cancer antigen 125 (CA - 125) is an important biomarker for the diagnosis of ovarian cancer. In this paper, oligonucleotide 5'-GACAGGCCCGAAGGAATAGATAATACGACTCACTATAGGGAGACAAGAATAAACGCTCAA-3' (oligo 1) contains an aptamer of CA - 125, and was designed partly complementary to oligonucleotide 5'-CTCTCTCTCCACCTTCTTCTTTGAGCGTTTATTCTTGTCT-3' (oligo 2). Oligo 1 · oligo 2 was extended with the Klenow fragment (exo-) polymerase for further polymerase chain reaction (PCR) processes in the presence of two primers: deoxyribose nucleoside triphosphate and Taq polymerase. Single-stranded DNA was produced at two sides of the PCR product by introducing a C18 spacer into the two primers, which could hybridize with AuNPs-DNA probes, investigated by dynamic light scattering and fluorescence. The addition of CA - 125 can interrupt the hybridization between oligo 1 and oligo 2, causing the average diameter of AuNPs-DNA probes to decrease with the increase of CA-125 within the range of 5 fg mL-1 - 50 ng mL-1. The linear regression equation of this relationship was D = 430.48-49.60 log10C, with a detection limit of 1.1 fg mL-1. Fluorescein molecules were modified at the end of the forward primer. The fluorescence intensity of the PCR product can be measured simultaneously, with the fluorescence intensity increasing linearly with the logarithm of CA-125 concentration within a linear range from 10 fg mL-1 to 50 ng mL-1, with a detection limit of 1.5 fg mL-1.

中文翻译：

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通过金纳米粒子探针识别聚合酶链反应产物对癌抗原125的动态光散射和荧光双信号传感

癌抗原 125 (CA-125) 是诊断卵巢癌的重要生物标志物。在本文中，寡核苷酸 5'-GACAGGCCCGAAGGAATAGATACGACTCACTATAGGGAGACAAGAATAAACGCTCAA-3'（寡核苷酸 1）包含一个 CA-125 适配体，设计为与寡核苷酸 5'-CTCTCTCTCCACCTTCTTTCTTTGAGCGTTTATTCTTGTCT2' (oligo 1) 部分互补。Oligo 1·oligo 2 用 Klenow 片段 (exo-) 聚合酶延伸，用于在两种引物存在下进一步聚合酶链反应 (PCR) 过程：脱氧核糖核苷三磷酸和 Taq 聚合酶。通过在两个引物中引入 C18 间隔区，在 PCR 产物的两侧产生单链 DNA，其可以与 AuNPs-DNA 探针杂交，通过动态光散射和荧光研究。CA-125的加入可以中断oligo 1和oligo 2之间的杂交，导致AuNPs-DNA探针的平均直径在5 fg mL-1 - 50 ng mL-1范围内随着CA-125的增加而减小. 这种关系的线性回归方程为 D = 430.48-49.60 log10C，检测限为 1.1 fg mL-1。在正向引物的末端修饰荧光素分子。可同时测量PCR产物的荧光强度，荧光强度在10 fg mL-1至50 ng mL-1的线性范围内随CA-125浓度的对数线性增加，检测限为1.5 fg毫升-1。这种关系的线性回归方程为 D = 430.48-49.60 log10C，检测限为 1.1 fg mL-1。在正向引物的末端修饰荧光素分子。可同时测量PCR产物的荧光强度，荧光强度在10 fg mL-1至50 ng mL-1的线性范围内随CA-125浓度的对数线性增加，检测限为1.5 fg毫升-1。这种关系的线性回归方程为 D = 430.48-49.60 log10C，检测限为 1.1 fg mL-1。在正向引物的末端修饰荧光素分子。可同时测量PCR产物的荧光强度，荧光强度在10 fg mL-1至50 ng mL-1的线性范围内随CA-125浓度的对数线性增加，检测限为1.5 fg毫升-1。