Rapid and quantitative determination of blood propofol concentration is important for anesthesiologists to accurately control intraoperative propofol dose, timely monitor physiological statuses of patients and greatly improve the safety of surgery. Herein, a dopant-assisted negative photoionization ion mobility spectrometer with the optimized ionization region structure and the three-way inlet design was developed, increasing the generation ratio of the reactant ions O2-, and improving the ionization efficiency of propofol molecules. Besides, the addition of methanol-anisole solution during injection promoted the neutral desorption of propofol in blood, further improving the detection sensitivity by an order of magnitude, eliminating any sample pretreatment and effectively reducing the single analysis time to less than 1 min compared to the previous article. The dual calibration quantitative method, i.e. the method of calibrating the O2- concentration and the sample concentration changes during the entire process of detecting propofol through the integral value of M·O2- and the maximum signal intensity of O2-, successfully achieved accurate quantification of blood propofol. And the linear calibration curve of propofol was obtained with the range of 0.1-15 ng μL-1 and with the limit of detection of 0.03 ng μL-1, which was fulfilled to conduct propofol determination throughout the perioperative period. Finally, this method was applied to clinically measure the blood propofol concentration in patients newly regained consciousness with concentrations ranging from 0.2 ng μL-1 to 3 ng μL-1, and it turned out that the older patient had the lower propofol concentration in blood.

中文翻译：

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使用带溶剂辅助中性解吸的负光电离离子迁移谱仪快速定量测定整个围手术期的血液丙泊酚浓度

快速定量测定血丙泊酚浓度对于麻醉医师准确控制术中丙泊酚剂量，及时监测患者生理状态，大大提高手术安全性具有重要意义。在此，开发了一种优化电离区结构和三路入口设计的掺杂剂辅助负光电离离子迁移谱仪，提高了反应物离子O2-的生成率，提高了丙泊酚分子的电离效率。此外，注射时加入甲醇-苯甲醚溶液促进了丙泊酚在血液中的中性解吸，进一步将检测灵敏度提高了一个数量级，与上一篇文章相比，无需任何样品预处理，有效地将单次分析时间缩短至 1 分钟以内。双标定定量法，即通过M·O2-的积分值和O2-的最大信号强度来标定丙泊酚检测全过程中O2-浓度和样品浓度变化的方法，成功实现了对丙泊酚的准确定量。血液丙泊酚。得到丙泊酚的线性校准曲线，范围为0.1～15 ng μL-1，检出限为0.03 ng μL-1，满足整个围手术期丙泊酚的测定。最后，将该方法应用于临床测量新恢复意识患者的血液丙泊酚浓度，浓度范围为0.