Abstract

Activity of digestive enzymes and certain biochemical parameters in the native and freeze-dried duodenal digesta and freeze-dried ileal digesta were studied in a chronic experiment in broiler chicks with chronic duodenal or ileal fistulae fed with various calcium doses. The results show that the amylase activity after freeze-drying in the control group increases 5.7-fold, the lipase activity 11.6-fold, and the activity of total proteases 8.2-fold. For alkaline phosphatase, the increase in activity is 15.9-fold. The calcium level after freeze drying increases 11.4-fold and the phosphorus content 17.7-fold. The cholesterol level after drying is 4.8-fold higher. As calcium levels in the diet of broilers decrease from 0.9 to 0.8%, the activity of alkaline phosphatase in the duodenal digesta increases by 35.1% compared with the control group (p ≤ 0.05). Therefore, freeze drying changes the enzymatic activity and the content of minerals in the chyme unevenly. When the level of calcium in the diet changes, the activity of amylase, lipase, proteases, alkaline phosphatase, calcium, phosphorus, and cholesterol in the ileal content is shown to adapt, which can be used to diagnose intestinal health of poultry in new test systems.

中文翻译：

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日粮钙与肉仔鸡消化酶活性的关系

摘要

在一项慢性实验中，对饲喂不同钙剂量的慢性十二指肠或回肠瘘的肉鸡进行了天然和冻干十二指肠消化物以及冻干回肠消化物中消化酶的活性和某些生化参数的研究。结果表明，对照组冷冻干燥后的淀粉酶活性增加了5.7倍，脂肪酶活性增加了11.6倍，总蛋白酶的活性增加了8.2倍。对于碱性磷酸酶，活性的增加是15.9倍。冷冻干燥后的钙含量增加了11.4倍，磷含量增加了17.7倍。干燥后的胆固醇水平高4.8倍。随着肉鸡日粮钙水平从0.9％降至0.8％，与对照组相比，十二指肠消化道中碱性磷酸酶的活性增加了35.1％（p ≤0.05）。因此，冷冻干燥不均匀地改变了食糜中的酶活性和矿物质含量。当饮食中的钙水平发生变化时，回肠内容物中的淀粉酶，脂肪酶，蛋白酶，碱性磷酸酶，钙，磷和胆固醇的活性就会显示出适应性，可以在新的测试中用于诊断家禽的肠道健康系统。