Abstract

A comparative analysis of population-genetic structures of three indigenous sheep breeds (Kalmyk, Edilbay, and Karachay breeds) and closely related wild species of the European Mouflon has been performed with polylocus genotyping at the highly polymorphic fragments of genomic DNA flanked by inverted repeats of microsatellite loci and a long terminal repeat region of endogenous retrovirus BERV-K1 (ISSR-PCR and IRAP-PCR markers). Increased genetic heterogeneity of ISSR-PCR and IRAP-PCR markers in males compared to females is revealed in all of the tested animal groups. The spectra of amplification products, including breed- and species-specific genomic DNA fragments, are identified. Genotyping for them may contribute to the control over the process of consolidation of breeds and intrabreed groups. Representatives of the European Mouflon are found to be different from the domesticated sheep in higher-rank polymorphism in the fragments of genomic DNA flanked by inverted repeats of BERV-K1, which may indicate the mobile genetic elements involved in the population-genetic differentiation between the domesticated and closely related wild species.

中文翻译：

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Mouflon与高度多态基因组元件中的家养绵羊品种之间的群体遗传关系

摘要

通过对基因座DNA的高度多态性片段侧翼为反向重复序列进行多位点基因分型，对欧洲Mouflon的三个本地绵羊品种（Kalmyk，Edilbay和Karachay品种）和密切相关的野生物种的种群遗传结构进行了比较分析。微卫星基因座和内源性逆转录病毒BERV-K1的长末端重复区域（ISSR-PCR和IRAP-PCR标记）。与所有雌性动物相比，雌性中ISSR-PCR和IRAP-PCR标记的遗传异质性增加。确定了扩增产物的光谱，包括特定品种和物种的基因组DNA片段。对它们进行基因分型可能有助于控制品种和种内群体的整合过程。