Symbionts participate in various physiological activities of their insect hosts, including detoxification metabolism. Emerging evidence has revealed that the bacterial symbiont Arsenophonus is involved in insecticide detoxification metabolism of Nilaparvata lugens, which harbors diverse symbionts. However, it is still unknown whether other bacterial symbionts have a functional role in this process. This study showed that pretreatment with antibiotics significantly increased N. lugens susceptibility to imidacloprid, chlorpyrifos, and clothianidin, and the detoxifying enzyme activities of the cytochrome P450 enzyme (P450) and glutathione S-transferase (GST) were significantly inhibited. Notably, the P450 genes NlCYP6ER1 and NlCYP4CE1, which are related to imidacloprid metabolism, were dramatically downregulated in ciprofloxacin- and tetracycline-pretreated N. lugens, respectively. Furthermore, the expression levels of various detoxifying genes (GSTs and P450s) were significantly positively correlated with Wolbachia, Arsenophonus, Acinetobacter, and Staphylococcus. These results indicated that bacterial symbionts may affect insecticide metabolism by regulating the expression of the insect host’s GST and P450 genes, and provide a foundation for further study on the mechanism of symbiont-mediated host detoxification metabolism in insect pests.

共生菌参与昆虫宿主的各种生理活动，包括排毒代谢。越来越多的证据表明，细菌共生体Arsenophonus参与了Nilaparvata lugens的杀虫剂解毒代谢，后者具有多种共生成分。但是，尚不清楚其他细菌共生体在此过程中是否具有功能性作用。这项研究表明，抗生素预处理显着提高了猪肺炎支原体对吡虫啉，毒死rif和布比尼丁的敏感性，并且细胞色素P450酶（P450）和谷胱甘肽S-转移酶（GST）的解毒酶活性受到明显抑制。值得注意的是P450基因NlCYP6ER1和NlCYP4CE1，这些都与吡虫啉代谢，显着被下调的ciprofloxacin-和四环素预处理的褐飞虱分别。此外，各种排毒基因（GSTs和P450s）的表达水平与Wolbachia，Arsenophonus，不动杆菌和葡萄球菌呈显着正相关。这些结果表明细菌共生体可能通过调节昆虫宿主的GST和P450的表达来影响杀虫剂的代谢。 基因，为进一步研究共生体介导的害虫宿主解毒代谢机理提供基础。