Fusarium stalk rot disease (FSR) of maize caused by Fusarium verticillioides (Sacc.) Nirenberg is becoming an important biotic production constraint in many of the major maize growing areas causing substantial yield losses. Inbreds are preferred as parents in hybrid development owing to homozygous nature and high heterotic ability. Double haploid (DH) technology has emerged as a significant milestone. A total of 339 DH lines were generated from two inbred lines, VL1043 (susceptible) and CM212 (resistant), through in vivo haploid induction method. The 339 DH lines along with parents were phenotyped for their response to the FSR at the College of Agriculture, V. C. Farm, Mandya, India during summer, kharif and rabi seasons of the 2019–2020. Best linear unbiased predictors (BLUPs) were estimated for the FSR disease scores over three seasons. A wide range of BLUP scores of three to nine indicated the presence of higher variation for response of DH lines to FSR disease. The higher estimates of standardized range (1.31) and phenotypic coefficient of variation (19.80) also displayed higher variability. Nine lines were moderately resistant and 188 exhibited moderately susceptible reaction. The distribution of DH lines was positively skewed (1.34) and platykurtic (2.31) which suggested complementary epistasis and involvement of large number of genes in the disease expression.

中文翻译：

---

轮枝镰刀菌引起的镰刀菌茎腐病玉米双单倍体系的遗传特征

由轮叶镰刀菌 (Sacc.) Nirenberg 引起的玉米镰刀菌茎腐病 (FSR) 正在成为许多主要玉米种植区的重要生物生产限制因素，导致大量产量损失。由于纯合性质和高杂种优势能力，近交成为杂交发育的亲本。双单倍体 (DH) 技术已成为一个重要的里程碑。通过体内单倍体诱导方法，从两个自交系VL1043（易感）和CM212（抗性）中产生了总共339个DH系。在 2019-2020 年夏季、哈里夫和狂犬病季节，在印度曼迪亚 VC 农场农业学院对 339 条 DH 品系及其亲本进行了表型分析。估计了三个季节 FSR 疾病评分的最佳线性无偏预测因子 (BLUP)。3 到 9 的大范围 BLUP 分数表明 DH 系对 FSR 疾病的反应存在更高的变异。标准化范围 (1.31) 和表型变异系数 (19.80) 的较高估计值也显示出较高的变异性。9 个品系中度抗性，188 个品系表现出中度敏感反应。DH 系的分布呈正偏态 (1.34) 和 platykurtic (2.31)，这表明在疾病表达中存在互补的上位性和大量基因的参与。9 个品系中度抗性，188 个品系表现出中度敏感反应。DH 系的分布呈正偏态 (1.34) 和 platykurtic (2.31)，这表明在疾病表达中存在互补的上位性和大量基因的参与。9 个品系中度抗性，188 个品系表现出中度敏感反应。DH 系的分布呈正偏态 (1.34) 和 platykurtic (2.31)，这表明在疾病表达中存在互补的上位性和大量基因的参与。