Naringin, a dietary flavonoid of citrus fruits, was encapsulated in biodegradable proteins of zein/casein biopolymers for its enhanced functional properties. The formulation conditions for the synthesis of colloidal particles were optimised by Response surface Methodology (RSM) and the levels were at zein/sodium casein ratio 1.75, pH 9 and naringin 8.7 mg/mL. The optimised nanoparticles showed the mean particle size of 234 nm, zeta potential of − 28.2 mV and a narrow size distribution with the encapsulation efficiency of 71 ± 2%. The physiochemical characteristics of the nanoparticles were analysed by SEM, FTIR, AFM, XRD and DSC techniques. The release kinetics study demonstrated that the release of naringin from the nanoparticles was pH dependent and had an appreciable amount of in vitro bioaccessibility. In 3T3-L1 cell culture study, the encapsulated naringin showed higher anti-adipogenic activity than the unencapsulated molecule by lowering the intracellular lipid accumulation. Further, the toxicity studies in Caco-2 cells indicated that the nanoparticles were free of any adverse effects. Collectively, naringin encapsulated colloidal formulation can be a promising delivery system in functional foods for varied health benefits.

Naringin是一种柑橘类水果的饮食类黄酮，由于其增强的功能特性，被包裹在玉米醇溶蛋白/酪蛋白生物聚合物的生物可降解蛋白中。通过响应表面方法（RSM）优化了合成胶体颗粒的配方条件，其含量为玉米蛋白/酪蛋白钠比为1.75，pH 9和柚皮苷为8.7 mg / mL。优化后的纳米颗粒的平均粒径为234 nm，ζ电势为-28.2 mV，粒径分布较窄，封装效率为71±2％。通过SEM，FTIR，AFM，XRD和DSC技术分析了纳米颗粒的理化特性。释放动力学研究表明，柚皮苷从纳米颗粒的释放是pH依赖性的，并且在体外具有可观的量生物可及性。在3T3-L1细胞培养研究中，通过降低细胞内脂质的积累，封装的柚皮苷比未封装的分子具有更高的抗脂肪形成活性。此外，在Caco-2细胞中的毒性研究表明，纳米颗粒没有任何不利影响。总的来说，柚皮苷封装的胶体制剂可以成为功能食品中有益健康的有希望的递送系统。