Identification of new sources of salinity-tolerant genotypes is prerequisite for rice breeding programs in different saline ecosystems. In the present experiment, we characterized 177 landraces from the Western Ghats of Karnataka under natural saline field conditions for two years using morpho-physiological and grain quality parameters. Significant variation was present among landraces for seedling stage and reproductive stage salinity tolerance. The nutrient composition analysis of grain revealed an increase in average grain protein and carbohydrate content under saline conditions. Evaluation of twenty-two SSR markers associated with the Saltol region validated RM140, RM287 and RM3412 as best markers to classify landraces for saline tolerance. Polymorphism Information Content and genetic diversity indices showed that the markers RM10748 and RM10864 were highly useful for distinguishing landraces. Further, to benefit the exploitation of heterosis, eleven maintainers were identified among tolerant landraces and these genotypes could be further developed into male sterile lines for production of salinity-tolerant rice hybrids. Comparison with ‘Pokkali’ for morpho-physiological traits along with molecular confirmation showed that the landraces ‘Doddabaikalu,’ Kalaadikonda,’ Gajagunda’ and ‘Anekombina batha’ were superior donors carrying genomic regions for salinity tolerance.

中文翻译：

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新的耐盐性遗传系的标记辅助鉴定及其在杂交水稻（Oryza sativa L.）开发中的应用分类

鉴定耐盐基因型的新来源是不同盐分生态系统水稻育种计划的先决条件。在本实验中，我们使用形态生理学和谷物质量参数在天然盐田条件下对来自卡纳塔克邦西高止山脉的 177 种地方品种进行了两年的表征。地方品种之间在幼苗期和生殖期耐盐性方面存在显着差异。谷物的营养成分分析表明，在盐渍条件下，平均谷物蛋白质和碳水化合物含量增加。对与 Saltol 地区相关的 22 个 SSR 标记的评估验证了 RM140、RM287 和 RM3412 作为对地方品种进行耐盐性分类的最佳标记。多态性信息含量和遗传多样性指数表明，标记RM10748和RM10864对区分地方品种非常有用。此外，为了有利于利用杂种优势，在耐受性地方品种中鉴定了 11 个保持系，这些基因型可以进一步开发成雄性不育系，用于生产耐盐杂交水稻。与“Pokkali”的形态生理特征以及分子确认的比较表明，地方品种“Doddabaikalu”、“Kalaadikonda”、“Gajagunda”和“Anekombina Batha”是具有耐盐性基因组区域的优良供体。在耐盐的地方品种中鉴定了 11 个保持系，这些基因型可以进一步开发成雄性不育系，用于生产耐盐杂交水稻。与“Pokkali”的形态生理特征以及分子确认的比较表明，地方品种“Doddabaikalu”、“Kalaadikonda”、“Gajagunda”和“Anekombina Batha”是具有耐盐性基因组区域的优良供体。在耐盐的地方品种中鉴定了 11 个保持系，这些基因型可以进一步开发成雄性不育系，用于生产耐盐杂交水稻。与“Pokkali”的形态生理特征以及分子确认的比较表明，地方品种“Doddabaikalu”、“Kalaadikonda”、“Gajagunda”和“Anekombina Batha”是具有耐盐性基因组区域的优良供体。