Sugar-enriched media are used to produce extracellular substances (ECS) by Lactobacillus plantarum WCSF1, with a focus on growing stages and carbon source substrates. Combination of size exclusion chromatography and ATR-FTIR spectroscopy provides physicochemical patterns of bulk ECS produced along culture growing time. Secreted biopolymers present polydisperse and high molecular weight distributions, with significant amounts of carbohydrates and proteins. Results, supported by a multivariate statistical analysis, enable to differentiate the macromolecular content of bacterial ECS along the growing stages regardless of the growing media, highlighting a higher production of proteinaceous materials compared to polysaccharides. At the end of the exponential phase, common exoproteins were present in all the tested sugar-enriched media such as transglycosylases between 20 and 35 kDa, a muropeptidase at 36.9 kDa and a cell wall hydrolase. Additionally, L. plantarum WCFS1 secretes ECS with a greater diversity of proteins, when growing in the sucrose-enriched media.

富含糖的培养基用于通过植物乳杆菌生产细胞外物质 (ECS)WCSF1，重点关注生长阶段和碳源基质。尺寸排阻色谱和 ATR-FTIR 光谱的组合提供了沿培养物生长时间产生的大量 ECS 的物理化学模式。分泌的生物聚合物呈现多分散和高分子量分布，含有大量碳水化合物和蛋白质。结果，在多元统计分析的支持下，无论生长介质如何，都能区分细菌 ECS 在生长阶段的大分子含量，突出显示与多糖相比，蛋白质材料的产量更高。在指数期结束时，所有测试的富含糖的培养基中都存在常见的外蛋白，例如 20 到 35 kDa 的转糖基酶、36.9 kDa 的多肽酶和细胞壁水解酶。当在富含蔗糖的培养基中生长时，植物乳杆菌WCFS1 分泌具有更多蛋白质多样性的 ECS。