Circular RNA (circRNA) is a novel member of endogenous noncoding RNAs with widespread distribution and diverse cellular functions. Recently, circRNAs have been identified for their enrichment and stability in exosomes. However, the roles of circRNAs from umbilical cord blood exosomes in gestational diabetes mellitus (GDM) occurrence and fetus growth remains poorly understood. In this study, we used microarray technology to construct a comparative circRNA profiling of umbilical cord blood exosomes between GDM patients and controls. We found the exosome particle size was larger, and the exosome concentration was higher in the GDM patients. A total of 88,371 circRNAs in umbilical cord blood exosomes from two groups were evaluated. Of these, 229 circRNAs were significantly up-regulated and 278 circRNAs were significantly down-regulated in the GDM patients. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) biological pathway analyses demonstrated that circRNA parental genes involved in the regulation of metabolic process, growth and development were significantly enriched, which are important in GDM development and fetus growth. Further circRNA/miRNA interactions analysis showed that most of the exosomal circRNAs harbored miRNA binding sites, and some miRNAs were associated with GDM. Collectively, these results lay a foundation for extensive studies on the role of exosomal circRNAs in GDM development and fetus growth.

中文翻译：

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正常和妊娠糖尿病患者的脐带血外泌体中的环状RNA表达谱。

环状RNA（circRNA）是内源性非编码RNA的新型成员，具有广泛的分布和多种细胞功能。最近，已鉴定出circRNA在外泌体中的富集和稳定性。然而，来自脐带血外来体的circRNA在妊娠糖尿病（GDM）发生和胎儿生长中的作用仍知之甚少。在这项研究中，我们使用微阵列技术构建了GDM患者和对照之间脐带血外泌体的circRNA比较谱。我们发现GDM患者的外泌体粒径较大，且外泌体浓度较高。评估了两组脐带血外泌体中的总共88,371个circRNA。这些，GDM患者中有229个circRNA显着上调，而有278个circRNA显着下调。基因本体论（GO）和《京都基因与基因组百科全书》（KEGG）生物途径分析表明，参与代谢过程，生长和发育调控的circRNA亲本基因显着丰富，这对GDM发育和胎儿生长非常重要。进一步的circRNA / miRNA相互作用分析表明，大多数外泌体circRNA都具有miRNA结合位点，并且某些miRNA与GDM相关。总的来说，这些结果为广泛研究外泌体circRNA在GDM发育和胎儿生长中的作用奠定了基础。基因本体论（GO）和《京都基因与基因组百科全书》（KEGG）生物途径分析表明，参与代谢过程，生长和发育调控的circRNA亲本基因显着丰富，这对GDM发育和胎儿生长非常重要。进一步的circRNA / miRNA相互作用分析表明，大多数外泌体circRNA都具有miRNA结合位点，并且某些miRNA与GDM相关。总的来说，这些结果为广泛研究外泌体circRNA在GDM发育和胎儿生长中的作用奠定了基础。基因本体论（GO）和《京都基因与基因组百科全书》（KEGG）生物途径分析表明，参与代谢过程，生长和发育调控的circRNA亲本基因显着丰富，这对GDM发育和胎儿生长非常重要。进一步的circRNA / miRNA相互作用分析表明，大多数外泌体circRNA都具有miRNA结合位点，并且某些miRNA与GDM相关。总的来说，这些结果为广泛研究外泌体circRNA在GDM发育和胎儿生长中的作用奠定了基础。一些miRNA与GDM相关。总的来说，这些结果为广泛研究外泌体circRNA在GDM发育和胎儿生长中的作用奠定了基础。一些miRNA与GDM相关。总的来说，这些结果为广泛研究外泌体circRNA在GDM发育和胎儿生长中的作用奠定了基础。