The purpose of the study was to evaluate the in vitro antibacterial effect of experimental eye drops with bacteriophages in elimination of Staphylococcus spp. isolated from dogs with bacterial conjunctivitis.. The bacterial material was collected from dogs with independent clinical signs of bacterial conjunctivitis. Staphylococcus spp. were identified by phenotypic and genotypic methods (MALDI-TOF MS mass spectrometry). Antibiotic resistance was determined by the disc-diffusion method. Phage activity (Plaque forming units, PFU) was determined on double-layer agar plates. Phages with lytic titres > 108 PFU were used to prepare eye drops. The stability of the antibacterial titre was evaluated for preparations stored in sealed bottles as well as after opening and reclosing. The tests confirmed the occurrence of Staphylococcus spp. strains as etiological agents of bacterial conjunctivitis in dogs. A high percentage of strains were resistant to more than three antibiotics. The experimental phage eye drops used in the study exhibited 100% efficacy in vitro against the tested Staphylococcus isolates. Particularly noteworthy is the long duration of activity and constant antibacterial lytic titre of ≥108 PFU/mL of two eye drop solutions, nos. 7 and 12, after the bottle had been opened (21 days) and after hermetically sealed packaging (28 days) at 4–8 °C. The results represent the first stage of research and require continuation in vivo. If positive effects are obtained in animals, the results can be used in applied research in humans and animals.

中文翻译：

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含有针对葡萄球菌属的噬菌体溶液的滴眼剂的体外功效。从患有细菌性结膜炎的狗身上分离出来


本研究的目的是评估含有噬菌体的实验滴眼液消除葡萄球菌的体外抗菌效果。从患有细菌性结膜炎的狗身上分离出来。细菌材料是从具有独立的细菌性结膜炎临床症状的狗身上收集的。葡萄球菌属通过表型和基因型方法（MALDI-TOF MS 质谱法）进行鉴定。抗生素耐药性通过纸片扩散法测定。在双层琼脂平板上测定噬菌体活性（噬菌斑形成单位，PFU）。使用裂解滴度为> 108 PFU 的噬菌体来制备滴眼剂。对储存在密封瓶中以及打开和重新封闭后的制剂的抗菌滴度稳定性进行了评估。测试证实了葡萄球菌属的存在。菌株作为狗细菌性结膜炎的病原体。高比例的菌株对三种以上抗生素具有耐药性。研究中使用的实验性噬菌体滴眼液在体外对测试的葡萄球菌分离株表现出 100% 的功效。特别值得注意的是两种滴眼液的长活性持续时间和恒定的抗菌溶解滴度≥108 PFU/mL。图 7 和 12，在 4–8 °C 下打开瓶子后（21 天）和密封包装后（28 天）。结果代表了研究的第一阶段，需要在体内继续进行。如果在动物身上获得积极效果，结果可用于人类和动物的应用研究。