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Quantitative and ultrasensitive detection of brombuterol by a surface-enhanced Raman scattering (SERS)-based lateral flow immunochromatographic assay (FLIA) using AgMBA@Au–Ab as an immunoprobe
Analyst ( IF 3.6 ) Pub Date : 2020-10-26 , DOI: 10.1039/d0an01949f Wen Huang 1, 2, 3, 4, 5 , Enhui Guo 1, 2, 3, 4, 5 , Jianguo Li 1, 2, 3, 4, 5 , Anping Deng 1, 2, 3, 4, 5
Analyst ( IF 3.6 ) Pub Date : 2020-10-26 , DOI: 10.1039/d0an01949f Wen Huang 1, 2, 3, 4, 5 , Enhui Guo 1, 2, 3, 4, 5 , Jianguo Li 1, 2, 3, 4, 5 , Anping Deng 1, 2, 3, 4, 5
Affiliation
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Brombuterol is a new emerging β-adrenergic agonist that has been used as an additive in animal feed to enhance the lean meat-to-fat ratio. Due to its potential harm to consumers, it is urgent to develop sensitive, simple and rapid analytical methods to monitor brombuterol residue. In this study, a competitive lateral flow immunochromatographic assay (FLIA) based on surface-enhanced Raman scattering (SERS) was developed for ultrasensitive quantitative determination of brombuterol in swine liver, pork and feed samples. Ag@Au core–shell bimetallic nanoparticles with the highest SERS enhancement were synthesized, characterized and used as the substrate for preparation of the immunoprobe AgMBA@Au–Ab, in which the Raman reporter mercaptobenzoic acid (MBA) was embedded between the core–shell layers and monoclonal antibodies against brombuterol were immobilized on the surfaces of nanoparticles. The presence of brombuterol was identified through a color change on testing lines. In addition, quantitative detection of brombuterol was achieved by measuring the characteristic Raman peak intensity of MBA in the immunoprobes captured by the coating antigen. The IC50 and limit of detection (LOD) of the SERS-based FLIA for brombuterol were 45 pg mL−1 and 0.11 pg mL−1, respectively. The recoveries of brombuterol from spiked samples were in the range of 87.27–100.16% with relative standard deviations of 1.29%–6.99% (n = 3). The proposed SERS-based LFIA was proven to be a feasible method for ultrasensitive and rapid detection of brombuterol and might be a platform for sensitive and rapid detection of a broad range of analytes in clinical, environmental and food analyses.
中文翻译：
使用AgMBA @ Au–Ab作为免疫探针，通过基于表面增强拉曼散射（SERS）的侧流免疫色谱分析（FLIA）定量和超灵敏地检测溴丁肌醇
Brombuterol是一种新兴的β-肾上腺素能激动剂，已被用作动物饲料的添加剂以提高瘦肉与脂肪的比例。由于其对消费者的潜在危害，迫切需要开发灵敏，简单和快速的分析方法来监测溴酚的残留量。在这项研究中，开发了一种基于表面增强拉曼散射（SERS）的竞争性侧流免疫色谱分析（FLIA），用于超灵敏定量测定猪肝，猪肉和饲料样品中的溴酚。具有最高SERS增强作用的Ag @ Au核壳双金属纳米粒子被合成，表征并用作制备免疫探针Ag MBA的底物@ Au–Ab，其中拉曼报道的巯基苯甲酸（MBA）被嵌入核-壳层之间，并且针对溴化呋喃的单克隆抗体被固定在纳米颗粒表面。通过检测线上的颜色变化确定了溴苯丁胺醇的存在。此外，通过测量被包被抗原捕获的免疫探针中MBA的特征性拉曼峰强度，可以实现对溴酚的定量检测。基于SERS的FLIA的溴甲烷的IC 50和检出限（LOD）分别为45 pg mL -1和0.11 pg mL -1。加标样品中的溴丁醇回收率在87.27–100.16％范围内，相对标准偏差为1.29％–6.99％（n= 3）。提议的基于SERS的LFIA被证明是一种超灵敏和快速检测细支布洛芬的可行方法，并且可能是灵敏和快速检测临床，环境和食品分析中各种分析物的平台。
更新日期：2020-11-04
中文翻译：
使用AgMBA @ Au–Ab作为免疫探针，通过基于表面增强拉曼散射（SERS）的侧流免疫色谱分析（FLIA）定量和超灵敏地检测溴丁肌醇
Brombuterol是一种新兴的β-肾上腺素能激动剂，已被用作动物饲料的添加剂以提高瘦肉与脂肪的比例。由于其对消费者的潜在危害，迫切需要开发灵敏，简单和快速的分析方法来监测溴酚的残留量。在这项研究中，开发了一种基于表面增强拉曼散射（SERS）的竞争性侧流免疫色谱分析（FLIA），用于超灵敏定量测定猪肝，猪肉和饲料样品中的溴酚。具有最高SERS增强作用的Ag @ Au核壳双金属纳米粒子被合成，表征并用作制备免疫探针Ag MBA的底物@ Au–Ab，其中拉曼报道的巯基苯甲酸（MBA）被嵌入核-壳层之间，并且针对溴化呋喃的单克隆抗体被固定在纳米颗粒表面。通过检测线上的颜色变化确定了溴苯丁胺醇的存在。此外，通过测量被包被抗原捕获的免疫探针中MBA的特征性拉曼峰强度，可以实现对溴酚的定量检测。基于SERS的FLIA的溴甲烷的IC 50和检出限（LOD）分别为45 pg mL -1和0.11 pg mL -1。加标样品中的溴丁醇回收率在87.27–100.16％范围内，相对标准偏差为1.29％–6.99％（n= 3）。提议的基于SERS的LFIA被证明是一种超灵敏和快速检测细支布洛芬的可行方法，并且可能是灵敏和快速检测临床，环境和食品分析中各种分析物的平台。
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