Background: Diosmetin (DIOS) is the aglycone of the flavonoid glycoside, diosmin, derived naturally from the leaves of the legume, Olea europaea, and Acacia farnesiana. It has potent anticancer activity against multiple forms of cancers. However, the role of DIOS in renal carcinoma and its mechanism of action remain unclear.

Objective: The purpose of this study is to investigate the effect of DIOS on cell viability and apoptosis in renal carcinoma cells and explore the possible mechanism of action.

Methods: Cell viability, cytotoxicity, caspase activity, apoptosis, and expression of apoptotic related proteins were analyzed in renal carcinoma ACHN cells.

Results: The results showed that DIOS inhibited the cell viability, and induced cytotoxicity and apoptosis in ACHN cells. Furthermore, DIOS increased expression of p53 mRNA and proteins, and downregulated phosphorylation of the phosphoinositide 3-kinase and protein B kinase (PI3K/AKT). In addition, it was observed that the anticancer effect of DIOS was significantly enhanced by the p53 activator, but inhibited by the p53 inhibitor.

Conclusion: Our data suggested that DIOS induced apoptosis in renal carcinoma ACHN cells by reducing AKT phosphorylation through p53 upregulation.

背景：薯os皂素（DIOS）是类黄酮苷，薯os皂甙的糖苷配基，得自豆类，欧洲油橄榄和相思树的叶子。它具有针对多种癌症的有效抗癌活性。但是，DIOS在肾癌中的作用及其作用机制仍不清楚。

目的：本研究的目的是研究DIOS对肾癌细胞的生存能力和凋亡的影响，并探讨其可能的作用机制。

方法：分析肾癌ACHN细胞的细胞活力，细胞毒性，胱天蛋白酶活性，凋亡和凋亡相关蛋白的表达。

结果：结果表明，DIOS抑制了ACHN细胞的细胞活力，并诱导了细胞毒性和凋亡。此外，DIOS增加了p53 mRNA和蛋白的表达，并下调了磷酸肌醇3激酶和蛋白B激酶（PI3K / AKT）的磷酸化。另外，观察到DIOS的抗癌作用被p53激活剂显着增强，但是被p53抑制剂抑制。

结论：我们的数据表明DIOS通过降低p53上调的AKT磷酸化来诱导肾癌ACHN细胞凋亡。