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Molecular, phylogenetic and antibiotic resistance analysis of enteroaggregative escherichia coli/uropathogenic Escherichia coli hybrid genotypes causing urinary tract infections
Indian Journal of Medical Microbiology ( IF 1.4 ) Pub Date : 2020-07-01 , DOI: 10.4103/ijmm.ijmm_20_365 Vinay Modgil 1 , Harpreet Kaur 1 , Balvinder Mohan 1 , Neelam Taneja 1
Background: Horizontal gene transfer of virulence genes (VGs) from different Escherichia coli pathotypes results in the evolution of hybrid strains. Hybrid genotypes of enteroaggregative E. coli and uropathogenic E. coli (EAEC/UPEC) have been reported in sporadic infections and outbreaks of extraintestinal origin. Yet, their association with routine infections is still underrated. Materials and Methods: In this study, we analysed 163 isolates of E. coli from cases of urinary tract infection seeking hybrid (EAEC/UPEC) strains. Using multiplex polymerase chain reaction, we investigated VGs (adhesive and toxin genes) of UPEC along with EAEC marker genes (aap and agg R), ast A (toxin genes) and serine protease autotransporters of Enterobacteriaceae, pet (plasmid-encoded toxin) and pic (mucinase gene). Those UPEC strains which had characteristic defining genes of EAEC (agg R/aap or their combination) were considered UPEC/EAEC hybrids. Results: Molecular predictors of EAEC (aap and aggR) were detected in 20.2% (33/163) of the strains. The pap C was also detected in 36% of the EAEC/UPEC hybrid strains. Phylogenetic analysis revealed that hybrid strains belonged to Group D (60.6%). Nearly 73.8% of UPEC and 75.7% of UPEC/EAEC hybrid strains were multidrug-resistant. Among UPEC isolates, 72.3% and in hybrid UPEC/EAEC, 78.7% isolates were able to produce biofilm. Conclusions: Our results indicated a closer relationship among EAEC and UPEC, which suggested that some EAEC strains can be potential uropathogens. Ours is a first study documenting the existence of EAEC pathotypes VGs in UPEC strains of nosocomial origin; further studies are required to understand the diarrhoeagenic potential of these hybrids.
中文翻译：
引起尿路感染的肠聚集性大肠杆菌/尿路致病性大肠杆菌杂种基因型的分子、系统发育和抗生素耐药性分析
背景：来自不同大肠杆菌致病型的毒力基因 (VG) 的水平基因转移导致杂交菌株的进化。据报道，肠道聚集性大肠杆菌和尿路致病性大肠杆菌(EAEC/UPEC) 的杂交基因型在散发性感染和肠外源暴发中出现。然而，它们与常规感染的关联仍被低估。材料和方法：在这项研究中，我们分析了 163 株来自尿路感染寻求杂种 (EAEC/UPEC) 菌株的大肠杆菌分离株。使用多重聚合酶链反应，我们研究了 UPEC 的 VG（粘附和毒素基因）以及 EAEC 标记基因（aap和agg R)、ast A（毒素基因）和肠杆菌科的丝氨酸蛋白酶自转运蛋白、pet（质粒编码的毒素）和pic（粘蛋白酶基因）。那些具有 EAEC 特征性定义基因（agg R/ aap或其组合）的UPEC 菌株被认为是 UPEC/EAEC 杂种。结果：在 20.2% (33/163) 的菌株中检测到EAEC 的分子预测因子（aap和aggR）。该PAP在 36% 的 EAEC/UPEC 杂种菌株中也检测到 C。系统发育分析表明，杂种菌株属于 D 组（60.6%）。近 73.8% 的 UPEC 和 75.7% 的 UPEC/EAEC 杂种菌株具有多重耐药性。在 UPEC 分离株中，72.3% 和在混合 UPEC/EAEC 中，78.7% 分离株能够产生生物膜。结论：我们的结果表明 EAEC 和 UPEC 之间的关系更密切，这表明一些 EAEC 菌株可能是潜在的泌尿病原体。我们的第一项研究记录了院内来源的 UPEC 菌株中存在 EAEC 病理型 VG；需要进一步研究以了解这些杂种的致泻潜力。
更新日期：2020-07-01
Indian Journal of Medical Microbiology ( IF 1.4 ) Pub Date : 2020-07-01 , DOI: 10.4103/ijmm.ijmm_20_365 Vinay Modgil 1 , Harpreet Kaur 1 , Balvinder Mohan 1 , Neelam Taneja 1
Affiliation
Background: Horizontal gene transfer of virulence genes (VGs) from different Escherichia coli pathotypes results in the evolution of hybrid strains. Hybrid genotypes of enteroaggregative E. coli and uropathogenic E. coli (EAEC/UPEC) have been reported in sporadic infections and outbreaks of extraintestinal origin. Yet, their association with routine infections is still underrated. Materials and Methods: In this study, we analysed 163 isolates of E. coli from cases of urinary tract infection seeking hybrid (EAEC/UPEC) strains. Using multiplex polymerase chain reaction, we investigated VGs (adhesive and toxin genes) of UPEC along with EAEC marker genes (aap and agg R), ast A (toxin genes) and serine protease autotransporters of Enterobacteriaceae, pet (plasmid-encoded toxin) and pic (mucinase gene). Those UPEC strains which had characteristic defining genes of EAEC (agg R/aap or their combination) were considered UPEC/EAEC hybrids. Results: Molecular predictors of EAEC (aap and aggR) were detected in 20.2% (33/163) of the strains. The pap C was also detected in 36% of the EAEC/UPEC hybrid strains. Phylogenetic analysis revealed that hybrid strains belonged to Group D (60.6%). Nearly 73.8% of UPEC and 75.7% of UPEC/EAEC hybrid strains were multidrug-resistant. Among UPEC isolates, 72.3% and in hybrid UPEC/EAEC, 78.7% isolates were able to produce biofilm. Conclusions: Our results indicated a closer relationship among EAEC and UPEC, which suggested that some EAEC strains can be potential uropathogens. Ours is a first study documenting the existence of EAEC pathotypes VGs in UPEC strains of nosocomial origin; further studies are required to understand the diarrhoeagenic potential of these hybrids.
中文翻译：
引起尿路感染的肠聚集性大肠杆菌/尿路致病性大肠杆菌杂种基因型的分子、系统发育和抗生素耐药性分析
背景：来自不同大肠杆菌致病型的毒力基因 (VG) 的水平基因转移导致杂交菌株的进化。据报道，肠道聚集性大肠杆菌和尿路致病性大肠杆菌(EAEC/UPEC) 的杂交基因型在散发性感染和肠外源暴发中出现。然而，它们与常规感染的关联仍被低估。材料和方法：在这项研究中，我们分析了 163 株来自尿路感染寻求杂种 (EAEC/UPEC) 菌株的大肠杆菌分离株。使用多重聚合酶链反应，我们研究了 UPEC 的 VG（粘附和毒素基因）以及 EAEC 标记基因（aap和agg R)、ast A（毒素基因）和肠杆菌科的丝氨酸蛋白酶自转运蛋白、pet（质粒编码的毒素）和pic（粘蛋白酶基因）。那些具有 EAEC 特征性定义基因（agg R/ aap或其组合）的UPEC 菌株被认为是 UPEC/EAEC 杂种。结果：在 20.2% (33/163) 的菌株中检测到EAEC 的分子预测因子（aap和aggR）。该PAP在 36% 的 EAEC/UPEC 杂种菌株中也检测到 C。系统发育分析表明，杂种菌株属于 D 组（60.6%）。近 73.8% 的 UPEC 和 75.7% 的 UPEC/EAEC 杂种菌株具有多重耐药性。在 UPEC 分离株中，72.3% 和在混合 UPEC/EAEC 中，78.7% 分离株能够产生生物膜。结论：我们的结果表明 EAEC 和 UPEC 之间的关系更密切，这表明一些 EAEC 菌株可能是潜在的泌尿病原体。我们的第一项研究记录了院内来源的 UPEC 菌株中存在 EAEC 病理型 VG；需要进一步研究以了解这些杂种的致泻潜力。
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