当前位置：
X-MOL 学术
›
Indian J. Med. Microbiol.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
A real-time polymerase chain reaction-based approach for qualitative estimation of viral RNA in organ tissues of coxsackievirus A-16-infected neonatal mice
Indian Journal of Medical Microbiology ( IF 1.4 ) Pub Date : 2020-07-01 , DOI: 10.4103/ijmm.ijmm_20_117 Sanjaykumar Sidram Tikute 1 , Shailesh Dattatraya Pawar 2 , Samruddhi Satish Kawale 2 , Deepa Kailash Sharma 2 , Varanasi Gopalkrishna 1
Hand, foot and mouth disease (HFMD) is a paediatric disease associated with enteroviruses (EVs). Among EVs, coxsackievirus A-16 (CVA-16) strain is currently in circulation and causing outbreaks in India. Neonatal mice (Institute of Cancer Research) strains were infected with CVA-16 strain isolated from HFMD patients to conduct pathological and molecular studies. Infected organs were harvested as per time points. A real-time polymerase chain reaction was used for qualitative estimation of viral RNA in organ tissues of infected mice. Skeletal muscle, brain tissue and cardiac tissues were the major target sites of CVA-16 tropism. The first-ever study was conducted on CVA-16 strains using the current approach in India.
中文翻译：
基于实时聚合酶链反应的方法定性评估柯萨奇病毒 A-16 感染的新生小鼠器官组织中的病毒 RNA
手足口病（HFMD）是一种与肠道病毒（EV）相关的儿科疾病。在电动汽车中，柯萨奇病毒 A-16 (CVA-16) 株目前正在传播并在印度引发疫情。新生小鼠（癌症研究所）品系感染手足口病患者分离的CVA-16品系，进行病理和分子研究。按照时间点收获感染的器官。使用实时聚合酶链反应对受感染小鼠器官组织中的病毒 RNA 进行定性评估。骨骼肌、脑组织和心脏组织是CVA-16趋向性的主要靶点。首次研究是在印度使用当前方法对 CVA-16 菌株进行的。
更新日期：2020-07-01
Indian Journal of Medical Microbiology ( IF 1.4 ) Pub Date : 2020-07-01 , DOI: 10.4103/ijmm.ijmm_20_117 Sanjaykumar Sidram Tikute 1 , Shailesh Dattatraya Pawar 2 , Samruddhi Satish Kawale 2 , Deepa Kailash Sharma 2 , Varanasi Gopalkrishna 1
Affiliation
Hand, foot and mouth disease (HFMD) is a paediatric disease associated with enteroviruses (EVs). Among EVs, coxsackievirus A-16 (CVA-16) strain is currently in circulation and causing outbreaks in India. Neonatal mice (Institute of Cancer Research) strains were infected with CVA-16 strain isolated from HFMD patients to conduct pathological and molecular studies. Infected organs were harvested as per time points. A real-time polymerase chain reaction was used for qualitative estimation of viral RNA in organ tissues of infected mice. Skeletal muscle, brain tissue and cardiac tissues were the major target sites of CVA-16 tropism. The first-ever study was conducted on CVA-16 strains using the current approach in India.
中文翻译：
基于实时聚合酶链反应的方法定性评估柯萨奇病毒 A-16 感染的新生小鼠器官组织中的病毒 RNA
手足口病（HFMD）是一种与肠道病毒（EV）相关的儿科疾病。在电动汽车中，柯萨奇病毒 A-16 (CVA-16) 株目前正在传播并在印度引发疫情。新生小鼠（癌症研究所）品系感染手足口病患者分离的CVA-16品系，进行病理和分子研究。按照时间点收获感染的器官。使用实时聚合酶链反应对受感染小鼠器官组织中的病毒 RNA 进行定性评估。骨骼肌、脑组织和心脏组织是CVA-16趋向性的主要靶点。首次研究是在印度使用当前方法对 CVA-16 菌株进行的。
京公网安备 11010802027423号