Abstract

We aimed to investigate the contribution of irisin in the neuroprotective process of exercise training in diabetic rats. Serum irisin levels, thermal and mechanical pain thresholds and intracellular calcium ([Ca²⁺]_i) levels in sensory neurons were measured at different time intervals during the eight weeks of exercise sessions for the control, non-exercise diabetics (3 groups) and exercise performing (low and high intensity groups) diabetic rats (n = 7-10 for all groups). Non-exercise diabetic groups were treated with irisin in different doses (1, 10 and 20 µg/kg respectively). Recovered pain thresholds at the end of the exercise sessions (p < .05), higher serum irisin levels that compared to control and diabetics (p < .05) and insignificant mean [Ca2⁺]_i peak amplitudes in sensory neurons (p > .05) obtained from experiments. Furthermore, irisin injection decreased the thermal pain threshold of diabetics only at 60th minutes (p < .05). Irisin may have a role in the neuroprotective effect of exercise training.

摘要

我们旨在研究鸢尾素在糖尿病大鼠运动训练的神经保护过程中的作用。血清鸢尾素水平、热痛阈值和机械痛阈值以及感觉神经元的细胞内钙 ([Ca ²⁺ ] _i ) 水平在八周的运动期间测量了对照组、非运动糖尿病患者（3 组）和运动表演（低强度和高强度组）糖尿病大鼠（所有组 n = 7-10）。非运动糖尿病组接受不同剂量的鸢尾素治疗（分别为 1、10 和 20 µg/kg）。在运动结束时恢复疼痛阈值 ( p  < .05)，与对照组和糖尿病患者相比，血清鸢尾素水平更高 ( p < .05) 和微不足道的平均值 [Ca2 ⁺ ] _{i从实验中获得的感觉神经元 (} p > .05)的峰值振幅 。此外，注射鸢尾素仅在第 60 分钟时降低了糖尿病患者的热痛阈值 ( p  < .05)。鸢尾素可能在运动训练的神经保护作用中发挥作用。