Abstract

Herniarin is a member of simple coumarins, which are a group of common secondary metabolites in plants. The aim of the present study was to investigate the effects of herniarin on genotoxicity and apoptosis induced by cisplatin in rat bone marrow cells. The experimental rats were treated with four different doses of herniarin (50, 100, 200, and 400 mg/kg.) for seven consecutive days. The cisplatin (5 mg/kg, i.p.) was injected into mice 1 h after the last oral herniarin administration on the seventh day. The protective effects of herniarin were investigated by hematological test, flow cytometry, micronucleus assay, and reactive oxygen species (ROS) level analysis. Herniarin caused a marked reduction in the frequencies of micronucleated polychromatic erythrocytes (MnPCEs) and micronucleated normochromatic erythrocytes (MnNCEs) 24 h after exposure to cisplatin at doses of 200 and 400 mg/kg. Furthermore, herniarin significantly increased the levels of both red and white blood cells in peripheral blood. Treatment of rats with herniarin before cisplatin, significantly decreased the percentage of apoptotic and necrotic cells and the ROS level in bone marrow cells. This study indicated that herniarin can be introduced as a new chemoprotective agent against cisplatin-induced genotoxicity in the future.

摘要

Herniarin 是简单香豆素的成员，香豆素是植物中一组常见的次生代谢产物。本研究的目的是研究疝气对顺铂诱导大鼠骨髓细胞的遗传毒性和细胞凋亡的影响。实验大鼠连续 7 天用四种不同剂量的疝气（50、100、200 和 400 mg/kg.）治疗。在第 7 天最后一次口服疝气给药后 1 小时将顺铂 (5 mg/kg, ip) 注射到小鼠体内。通过血液学试验、流式细胞术、微核试验和活性氧 (ROS) 水平分析研究疝气的保护作用。在以 200 和 400 mg/kg 剂量暴露于顺铂后 24 小时，疝气引起微核多染红细胞 (MnPCE) 和微核正常染色红细胞 (MnNCE) 的频率显着降低。此外，疝气显着增加外周血中红细胞和白细胞的水平。在顺铂之前用疝气治疗大鼠，显着降低了凋亡和坏死细胞的百分比以及骨髓细胞中的活性氧水平。该研究表明，疝气在未来可以作为一种新的化学保护剂被引入，以对抗顺铂诱导的基因毒性。显着降低了骨髓细胞中凋亡和坏死细胞的百分比和ROS水平。该研究表明，疝气在未来可以作为一种新的化学保护剂被引入，以对抗顺铂诱导的基因毒性。显着降低了骨髓细胞中凋亡和坏死细胞的百分比和ROS水平。该研究表明，疝气在未来可以作为一种新的化学保护剂被引入，以对抗顺铂诱导的基因毒性。