Abnormal increases in reactive oxygen species in dying insects infected with nematodes,Archives of Insect Biochemistry and Physiology

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Abnormal increases in reactive oxygen species in dying insects infected with nematodes
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2020-11-03 , DOI: 10.1002/arch.21758
Urara Tonogawa ₁ , Takashi Matsumura _{1,

2} , Masaya Ono _{1,

2} , Toyoshi Yoshiga _{1,

2}

Affiliation

Stress enhances the concentration of reactive oxygen species (ROS) in animal plasma. Increased ROS alter various physiological functions, such as development and the immune response, but excessive increases could be harmful. In this study, we tested the hypothesis that abnormally increased plasma ROS levels are associated with animal death. Injection of the nematode Caenorhabditis elegans into insect larvae caused high mortality in Galleria mellonella, and the plasma ROS concentration was four times higher than M9 buffer‐injected larvae. There was no difference in plasma antioxidant activity after nematode injection. However, coinjecting nematodes with an antioxidant (ascorbic acid or N‐acetylcysteine) suppressed increases in ROS concentrations by the nematodes and increases in the number of nematodes in the larvae, which increased G. mellonella survival. These results suggest that the abnormal elevation of ROS associated with the stress caused by nematode propagation is lethal for G. mellonella.

中文翻译：

线虫感染垂死昆虫中活性氧的异常增加

应激会增加动物血浆中活性氧（ROS）的浓度。ROS增加会改变各种生理功能，例如发育和免疫反应，但过度增加可能有害。在这项研究中，我们测试了血浆ROS水平异常升高与动物死亡相关的假设。将线虫秀丽隐杆线虫注射到昆虫幼虫中导致了梅勒内尔拱廊的高死亡率，并且血浆ROS浓度是M9缓冲液注射幼虫的四倍。线虫注射后血浆抗氧化剂活性没有差异。但是，将线虫与抗氧化剂（抗坏血酸或N-乙酰半胱氨酸）抑制了线虫的ROS浓度增加以及幼虫中线虫的数量增加，从而增加了G. mellonella的存活率。这些结果表明，与应激有关ROS的异常升高引起的线虫传播是致死为蜡螟。

更新日期：2020-11-03

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