Electrical stimulation has shown great potential for nerve regeneration processes. This makes it attractive to use electrically active materials in the neural scaffold. In this paper, bismuth ferrite (BFO) nanoparticles were synthesized via co‐precipitation method and incorporated to 10 wt% polylactic acid (PLA) in chloroform to obtain 3D‐printed PLA/BFO biocomposites. The crystallinity of BFO nanoparticles was confirmed by XRD, and we studied its chemical structure with FTIR, as well as the mechanical properties of the 3D‐printed composites. in vitro studies show that 3D‐printed scaffolds have no cytotoxicity and support the proliferation of human adipose‐derived mesenchymal stem cells (hADMSCs). Furthermore, 3D scaffolds embedded with BFO shows the highest cell viability relative to pristine PLA and BFO‐lined PLA scaffolds. A 48 hours electrical stimulation on the hADMSC cultured inside the 3D‐printed BFO‐lined PLA scaffolds indicates that stimulated cells are aligned toward the BFO line. These results could indicate the potential of BFO for directing cells toward damaged tissues.

中文翻译：

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电刺激对3D打印支架中培养的人脂肪间充质干细胞的影响

电刺激已显示出神经再生过程的巨大潜力。这使得在神经支架中使用电活性材料具有吸引力。在本文中，通过共沉淀法合成了铁酸铋（BFO）纳米颗粒，并将其掺入氯仿中的10 wt％聚乳酸（PLA）中以获得3D打印的PLA / BFO生物复合材料。XRD证实了BFO纳米颗粒的结晶度，我们用FTIR研究了其化学结构以及3D打印复合材料的机械性能。体外研究表明3D打印的支架没有细胞毒性，并支持人脂肪来源的间充质干细胞（hADMSCs）的增殖。此外，相对于原始的PLA和BFO衬里的PLA支架，嵌入BFO的3D支架显示出最高的细胞活力。在3D打印的BFO衬里的PLA支架内培养的hADMSC上进行48小时的电刺激，表明刺激的细胞朝向BFO线排列。这些结果可能表明BFO可能将细胞导向受损组织。