Hydrogen peroxide (H₂O₂) is used to treat sea lice infections of farmed salmonids in the Atlantic and Pacific Oceans and issues with resistance to this treatment, and others are a major threat to the sustainability of the industry. The objectives of this study were to determine how H₂O₂ exposure affects survival and antioxidant‐related gene expression in salmon lice (Lepeophtheirus salmonis) collected from the Bay of Fundy, New Brunswick. The maximum recommended dose of H₂O₂ is 1,800 mg/L, while the EC₅₀ values (with 95% CI) for the population tested were 1,486 (457, 2,515) mg/L for males and 2,126 (984, 3,268) mg/L for females. Neither temperature nor pretreatment with emamectin benzoate (EMB) impacted survival after H₂O₂ exposure. RT‐qPCR was performed on pre‐adult sea lice exposed to H₂O₂ and showed that four genes classically involved in the response to oxidative stress were unchanged between treated and control groups. Seven genes were found to be significantly upregulated in males and one in females. This is the first report on the efficacy and molecular responses of Atlantic Canada sea lice to H₂O₂ treatment.

中文翻译：

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过氧化氢处理及其对源自加拿大芬迪湾的鲑鱼Leophophtheirus鲑鱼的影响

过氧化氢（H ₂ O ₂）用于治疗大西洋和太平洋养殖的鲑鱼的海虱感染以及这种治疗的抗性问题，而其他问题则对该行业的可持续发展构成重大威胁。这项研究的目的是确定H ₂ O ₂暴露如何影响从新不伦瑞克省芬迪湾采集的鲑鱼虱（Lepeophtheirus鲑鱼）的存活和抗氧化剂相关基因的表达。H ₂ O ₂的最大推荐剂量为1,800 mg / L，而EC ₅₀男性的测试值（具有95％的CI）为1,486（457，2,515）mg / L，女性为2126（984，3,268）mg / L。暴露于H ₂ O ₂后，温度或苯甲酸氨连环素（EMB）的预处理均不影响生存。在暴露于H ₂ O ₂的成年海虱上进行了RT-qPCR ，结果表明，治疗组和对照组之间四个经典参与氧化应激反应的基因没有变化。发现有七个基因在男性中显着上调，在女性中一个上调。这是关于加拿大大西洋海虱对H ₂ O ₂处理的功效和分子反应的第一份报告。