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CYP154C5 Regioselectivity in Steroid Hydroxylation Explored by Substrate Modifications and Protein Engineering**
ChemBioChem ( IF 2.6 ) Pub Date : 2020-11-04 , DOI: 10.1002/cbic.202000735
Paula Bracco 1 , Hein J Wijma 2 , Bastian Nicolai 1 , Jhon Alexander Rodriguez Buitrago 3 , Thomas Klünemann 4 , Agustina Vila 1 , Patrick Schrepfer 3 , Wulf Blankenfeldt 3, 4 , Dick B Janssen 2 , Anett Schallmey 3
Affiliation  

Changes in selectivity: Using substrate and protein engineering based on key residues and functional groups, the regioselectivity of the bacterial cytochrome P450 monooxygenase CYP154C5 in the hydroxylation of different steroids was altered. Apart from 16α‐hydroxylation, steroid products containing hydroxy groups in 15α and 21 position were formed.
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中文翻译:

通过底物修饰和蛋白质工程探索类固醇羟基化中的 CYP154C5 区域选择性**

选择性的变化:使用基于关键残基和官能团的底物和蛋白质工程,改变了细菌细胞色素 P450 单加氧酶 CYP154C5 在不同类固醇羟基化中的区域选择性。除了 16α-羟基化外,还形成了 15α 和 21 位含有羟基的类固醇产物。
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更新日期:2020-11-04
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