Silkworm sericin has been widely exploited in biomaterials due to its favorable biological activities. However, the extraction processes of sericin from silkworm cocoons can alter the biological and biophysical properties, including a structural diversity of natural sericin. In addition, extracted natural sericin is often contaminated with fibroin that may be harmful to human cells. Induction of tolerogenic dendritic cell (DC) has become a strategy in biomaterial fields because this cell type plays a key role in immune modulation and wound healing. To overcome undesired effects of extracted natural sericin and to improve its biological properties, we biosynthesized sericin 1-like protein that contained only functional motifs and tested its biological activity and immunomodulatory properties in fibroblasts and DCs, respectively. In comparison to natural sericin, biosynthetic sericin 1 promoted collagen production in fibroblasts at a late time point. Furthermore, DCs treated with biosynthetic sericin 1 exhibited a tolerogenic-like phenotype with semimaturation and low production of proinflammatory cytokines, but high production of anti-inflammatory cytokine, IL-10. Biosynthetic sericin 1 might be developed as immunomodulator or immunosuppressant.

中文翻译：

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生物合成丝胶 1 样蛋白使树突细胞偏向致耐受样表型

蚕丝胶蛋白因其良好的生物活性而被广泛用于生物材料。然而，从蚕茧中提取丝胶的过程可以改变生物和生物物理特性，包括天然丝胶的结构多样性。此外，提取的天然丝胶经常被丝心蛋白污染，可能对人体细胞有害。诱导耐受性树突状细胞 (DC) 已成为生物材料领域的一种策略，因为这种细胞类型在免疫调节和伤口愈合中起关键作用。为了克服提取的天然丝胶的不良影响并改善其生物学特性，我们生物合成了仅包含功能基序的丝胶 1 样蛋白，并分别测试了其在成纤维细胞和 DC 中的生物学活性和免疫调节特性。与天然丝胶相比，生物合成丝胶 1 在较晚的时间点促进了成纤维细胞中胶原蛋白的产生。此外，用生物合成丝胶蛋白 1 处理的 DCs 表现出类耐受性表型，具有半成熟和低促炎细胞因子产量，但抗炎细胞因子 IL-10 产量高。生物合成丝胶蛋白 1 可能被开发为免疫调节剂或免疫抑制剂。