CRISPR/Cas9: A new tool for the study and control of helminth parasites,BioEssays

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CRISPR/Cas9: A new tool for the study and control of helminth parasites
BioEssays ( IF 3.2 ) Pub Date : 2020-11-04 , DOI: 10.1002/bies.202000185
Xiaofeng Du _{1,

2} , Donald P McManus _{1,

2} , Juliet D French ₃ , Malcolm K Jones ₄ , Hong You ₁

Affiliation

Recent reports of CRISPR/Cas9 genome editing in parasitic helminths open up new avenues for research on these dangerous pathogens. However, the complex morphology and life cycles inherent to these parasites present obstacles for the efficient application of CRISPR/Cas9‐targeted mutagenesis. This is especially true with the trematode flukes where only modest levels of gene mutation efficiency have been achieved. Current major challenges in the application of CRISPR/Cas9 for study of parasitic worms thus lie in enhancing gene mutation efficiency and overcoming issues involved in host passage so that mutated parasites survive. Strategies developed for CRISPR/Cas9 studies on Caenorhabditis elegans, protozoa and mammalian cells, including novel delivery methods, the choice of selectable markers, and refining mutation precision represent novel tactics whereby these impediments can be overcome. Furthermore, employing CRISPR/Cas9‐mediated gene drive to interfere with vector transmission represents a novel approach for the control of parasitic worms that is worthy of further exploration.

中文翻译：

CRISPR/Cas9：研究和控制蠕虫寄生虫的新工具

最近关于寄生虫中 CRISPR/Cas9 基因组编辑的报告为研究这些危险病原体开辟了新途径。然而，这些寄生虫固有的复杂形态和生命周期为 CRISPR/Cas9 靶向诱变的有效应用带来了障碍。对于吸虫吸虫来说尤其如此，其中仅实现了中等水平的基因突变效率。因此，目前应用 CRISPR/Cas9 研究寄生虫的主要挑战在于提高基因突变效率和克服宿主传代所涉及的问题，以便突变的寄生虫存活。为秀丽隐杆线虫CRISPR/Cas9 研究制定的策略、原生动物和哺乳动物细胞，包括新的递送方法、可选择标记的选择和改进突变精度，代表了可以克服这些障碍的新策略。此外，采用 CRISPR/Cas9 介导的基因驱动来干扰载体传输是一种控制寄生虫的新方法，值得进一步探索。

更新日期：2020-12-22

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