East Coast fever (ECF) is an often fatal, economically important cattle disease that predominantly affects eastern, central, and southern Africa. ECF is controlled through vaccination by means of simultaneous injection of oxytetracycline and cryogenically preserved stabilate containing live, disease-causing parasites. Storage and transportation of the stabilate requires liquid nitrogen, a commodity that is commonly unreliable in low-resource settings. Here we show that storage of conventionally prepared stabilate at −80 °C for up to 30 days does not significantly affect its ability to infect cultured peripheral blood mononucleated cells or live cattle, suggesting an alternative cold chain that maintains these temperatures could be used to effectively manage ECF.

东海岸热病（ECF）是一种通常致命的，在经济上很重要的牛病，主要影响东部，中部和南部非洲。通过同时注射土霉素和低温保存的含有活的，引起疾病的寄生虫的羟苯甲酸，通过接种疫苗控制ECF。硬脂酸酯的储存和运输需要液氮，这是在资源贫乏地区通常不可靠的商品。在这里，我们表明，常规制备的羟基苯甲酸酯在-80°C下保存长达30天不会显着影响其感染培养的外周血单核细胞或活牛的能力，这表明维持这些温度的替代冷链可用于有效地管理ECF。