Liver malignant growth incorporates hepatocellular carcinoma (HCC) involving 75 %–85 % of liver patients respectively which is attributed mainly to the lack of early biomarker/s and poor accesses to affordable, simple diagnostic techniques, which is not available in the conventional techniques of HCC diagnosis. Consequently, there is an urgent need to develop an alternative simple, rapid HCC diagnostic system. One side of this development is the use of a lab on a chip, which facilitates the detection of cancer cells quickly and efficiently at a lower cost. Dielectrophoresis (DEP) phenomenon is the motion of neutral particles under the effect of the non-uniform electric field. DEP phenomenon is a widely used technique for manipulation and separation of biological cells. The proposed electro-kinetic platform is designed based on travelling wave dielectrophoresis (twDEP) configuration, which is implemented using a printed circuit board (PCB) technology. Herein, we have developed an electro-kinetic platform based on PCB technology to characterize, identify, and detect human liver cancer cells from clinical samples, compared to human normal liver cells. Also, rat’s normal muscle cells as a different organism has been tested. The experimental results proved the ability of the developed electro-kinetic platform to differentiate efficiently between HCC and normal liver cells from real clinical samples, and Rat’s normal muscle cells, with high specificity, accuracy and sensitivity.

肝恶性生长合并有肝细胞癌（HCC），分别涉及75％至85％的肝病患者，这主要是由于缺乏早期生物标志物和难以获得可负担得起的简单诊断技术，而常规肝癌的常规技术尚无此方法。 HCC诊断。因此，迫切需要开发另一种简单，快速的HCC诊断系统。这一发展的一个方面是使用芯片实验室，这有助于以较低的成本快速有效地检测癌细胞。介电泳（DEP）现象是中性粒子在非均匀电场作用下的运动。DEP现象是用于操纵和分离生物细胞的一种广泛使用的技术。所提出的电动平台是基于行波介电电泳（twDEP）配置设计的，该配置是使用印刷电路板（PCB）技术实现的。在此，我们已经开发了一种基于PCB技术的电动平台，与人类正常肝细胞相比，该平台可从临床样品中表征，鉴定和检测人类肝癌细胞。同样，已经测试了大鼠正常肌肉细胞作为另一种生物。实验结果证明了所开发的电动平台具有以高特异性，准确性和敏感性高效地区分真实临床样本中的HCC和正常肝细胞以及大鼠正常肌肉细胞的能力。我们已经开发了一种基于PCB技术的电动平台，用于与人正常肝细胞相比，从临床样品中表征，鉴定和检测人肝癌细胞。同样，已经测试了大鼠正常肌肉细胞作为另一种生物。实验结果证明了所开发的电动平台具有以高特异性，准确性和敏感性高效地区分真实临床样本中的HCC和正常肝细胞以及大鼠正常肌肉细胞的能力。我们已经开发了一种基于PCB技术的电动平台，用于与人正常肝细胞相比，从临床样品中表征，鉴定和检测人肝癌细胞。同样，已经测试了大鼠正常肌肉细胞作为另一种生物。实验结果证明了所开发的电动平台具有以高特异性，准确性和敏感性高效地区分真实临床样本中的HCC和正常肝细胞以及大鼠正常肌肉细胞的能力。