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Stereological investigation of 5-HT3 receptors in the substantia nigra and dorsal raphe nucleus in the rat
Journal of Chemical Neuroanatomy ( IF 2.7 ) Pub Date : 2021-01-01 , DOI: 10.1016/j.jchemneu.2020.101881
Sébastien Belliveau 1 , Woojin Kang 1 , Samantha Bovaird 2 , Adjia Hamadjida 1 , Dominique Bédard 1 , Numa Dancause 3 , Thomas Stroh 1 , Philippe Huot 4
Affiliation  

Serotonin (5-HT) is a common neurotransmitter in mammals, playing a central role in the regulation of various processes such as sleep, perception, cognitive and autonomic functions in the nervous system. Previous studies have demonstrated that 5-HT type 3 (5-HT3) receptors are expressed in either or both the substantia nigra (SN) and the dorsal raphe nucleus (DRN) in humans, marmosets, rats and Syrian hamsters. Here, we quantify the distribution of 5-HT3 receptors across these regions in the adult rat. Fluorescent immunohistochemistry was performed on sections of rat brain covering the entire rostro-caudal extent of the SN and DRN with antibodies specific to the 5-HT3A receptor subunit, as well as others targeting the monoaminergic markers tyrosine hydroxylase (TH) and the 5-HT transporter (SERT). The number of 5-HT3A receptor-positive, TH-positive (n = 28,428 ± 888, Gundersen's m = 1 coefficient of error [CE] = 0.05) and SERT-positive (n = 12,852 ± 462, CE = 0.06) cells were estimated in both the SN and the DRN using stereology. We found that 5-HT3A receptor-positive cells are present in the SNr (n = 1,250 ± 64, CE = 0.24), but they did not co-localise with TH-positive cells, nor were they present in the SNc. In contrast, no 5-HT3A receptor-positive cells were found in the DRN. These results support the presence of 5-HT3 receptors in the SN, but not in the DRN, and do not support their expression on monoaminergic cells within these two brain areas.

中文翻译:

大鼠黑质和中缝背核5-HT3受体的体视学研究

血清素 (5-HT) 是哺乳动物常见的神经递质,在调节神经系统的睡眠、感知、认知和自主神经功能等各种过程中发挥核心作用。先前的研究表明,5-HT 3 型 (5-HT3) 受体在人类、狨猴、大鼠和叙利亚仓鼠的黑质 (SN) 和中缝背核 (DRN) 中的一个或两个中表达​​。在这里,我们量化了 5-HT3 受体在成年大鼠中这些区域的分布。使用针对 5-HT3A 受体亚基的抗体以及其他靶向单胺能标记酪氨酸羟化酶 (TH) 和 5-HT 的抗体,对覆盖 SN 和 DRN 的整个 rostro-caudal 范围的大鼠大脑切片进行荧光免疫组织化学转运(SERT)。5-HT3A受体阳性的数量,TH 阳性(n = 28,428 ± 888,Gundersen's m = 1 误差系数 [CE] = 0.05)和 SERT 阳性(n = 12,852 ± 462,CE = 0.06)细胞在 SN 和 DRN 中均使用体视学进行估计. 我们发现 SNr 中存在 5-HT3A 受体阳性细胞(n = 1,250 ± 64,CE = 0.24),但它们不与 TH 阳性细胞共定位,也不存在于 SNc 中。相反,在 DRN 中没有发现 5-HT3A 受体阳性细胞。这些结果支持 SN 中存在 5-HT3 受体,但不支持 DRN,并且不支持它们在这两个大脑区域内的单胺能细胞上表达。我们发现 SNr 中存在 5-HT3A 受体阳性细胞(n = 1,250 ± 64,CE = 0.24),但它们不与 TH 阳性细胞共定位,也不存在于 SNc 中。相反,在 DRN 中没有发现 5-HT3A 受体阳性细胞。这些结果支持 SN 中存在 5-HT3 受体,但不支持 DRN,并且不支持它们在这两个大脑区域内的单胺能细胞上表达。我们发现 SNr 中存在 5-HT3A 受体阳性细胞(n = 1,250 ± 64,CE = 0.24),但它们不与 TH 阳性细胞共定位,也不存在于 SNc 中。相反,在 DRN 中没有发现 5-HT3A 受体阳性细胞。这些结果支持 SN 中存在 5-HT3 受体,但不支持 DRN,并且不支持它们在这两个大脑区域内的单胺能细胞上表达。
更新日期:2021-01-01
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