Alamandine (Ala¹-Arg²-Val³-Tyr⁴-Ile⁵-His⁶-Pro⁷), a heptapeptide hormone of the renin–angiotensin system (RAS), exerts its effects through the Mas-related G-protein coupled receptor of the type D, MrgD, which is expressed in different tissues, including the brain. In the present study, we tested the hypothesis that alamandine could attenuate the depression-like behavior observed in transgenic rats with low brain angiotensinogen, TGR (ASrAOGEN)680. Transgenic rats exhibited a significant increase in the immobility time in forced swim test, a phenotype reversed by intracerebroventricular infusion of alamandine. Pretreatment with D-Pro⁷-Ang-(1–7), a Mas/MrgD receptor antagonist, prevented the antidepressant-like effect induced by this peptide demonstrating, for the first time, that alamandine through MrgD receptor, can modulate depression-like behavior in TGR (ASrAOGEN)680. This result shows an action of alamandine which strengthens the importance of the counter-regulatory arms of the RAS in fight and treatment of neuropsychiatric diseases.

阿拉曼汀（Ala ¹ -Arg ² -Val ³ -Tyr ⁴ -Ile ⁵ -His ⁶ -Pro ⁷）是肾素-血管紧张素系统（RAS）的七肽激素，通过与Mas相关的G蛋白偶联受体发挥作用D型MrgD，在包括大脑在内的不同组织中表达。在本研究中，我们测试了以下假设：阿拉曼丁可以减轻在低脑血管紧张素原TGR（ASrAOGEN）680的转基因大鼠中观察到的抑郁样行为。转基因大鼠在强迫游泳试验中显示出不动时间的显着增加，这种表型被脑室内注入金刚烷胺逆转。使用D-Pro ^7进行预处理-Ang-（1-7），一种Mas / MrgD受体拮抗剂，阻止了由该肽诱导的抗抑郁样作用，这首次证明了通过MrgD受体的金刚烷胺可以调节TGR（ASrAOGEN）中的抑郁样行为680。该结果显示了阿拉曼丁的作用，其增强了RAS的反调节臂在对抗和治疗神经精神疾病中的重要性。