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Identification and expression of chemosensory receptor genes in the egg parasitoid Trissolcus basalis
Comparative Biochemistry and Physiology D: Genomics & Proteomics ( IF 2.2 ) Pub Date : 2020-11-04 , DOI: 10.1016/j.cbd.2020.100758
Huayan Chen 1 , Zachary Lahey 2 , Elijah J Talamas 3 , Norman F Johnson 4
Affiliation  

Parasitic wasps largely rely on chemosenses to locate resources. Understanding the evolution of their chemoreceptors can help elucidate the mechanisms underlying host adaptation and speciation. Trissolcus basalis is a biological control agent of the southern green stink bug, a pantropical pest, and is ideal for investigating the evolution of chemoreceptors. We identified 34 gustatory receptors, 170 odorant receptors, one odorant co-receptor, and 23 ionotropic receptors. Comparison with other Hymenoptera revealed species-specific expansions of 21 Grs and 53 Ors. Most of these Or expansions have 9 exons. Gender- and tissue-specific analyses showed that 5 Grs and 54 Ors are expressed only in antennae in both sexes, 66 Ors in female antennae only, and 4 Ors in male antennae alone. The identification and expression profile of chemosensory receptor genes in T. basalis helps in understanding the link between the evolution of chemoreceptors and speciation in parasitic wasps.



中文翻译:

卵寄生Trissolcus basalis化学感应受体基因的鉴定与表达

寄生蜂主要依靠化学感应器来定位资源。了解它们的化学感受器的进化有助于阐明宿主适应和物种形成的潜在机制。三足草是南方绿臭虫(一种泛热带害虫)的生物防治剂,是研究化学感受器进化的理想选择。我们确定了 34 种味觉受体、170 种气味受体、一种气味共受体和 23 种离子型受体。与其他膜翅目的比较揭示了 21 Grs 和 53 Ors 的物种特异性扩增。大多数这些 Or 扩展都有 9 个外显子。性别和组织特异性分析表明,5 Grs 和 54 Ors 仅在两性的触角中表达,66 Ors 仅在雌性触角中表达,4 Ors 仅在雄性触角中表达。T中化学感应受体基因的鉴定和表达谱。basalis有助于了解化学感受器的进化与寄生蜂的物种形成之间的联系。

更新日期:2020-11-15
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