Synechocystis sp. PCC 7338 (hereafter referred to as Synechocystis 7338) is a marine cyanobacterium that has the potential to produce photosynthetic pigments. In this study, we investigated the effects of various NaCl concentrations (0, 0.4, 0.8, and 1.2 M) on cell growth, photosynthetic pigments, and metabolites and intact lipid species profiles in Synechocystis 7338. The overall growth pattern of Synechocystis 7338 was similar under 0, 0.4, and 0.8 M NaCl conditions. Cell growth was retarded after reaching the exponential phase under 1.2 M NaCl; however, a similar growth pattern was observed after the exponential phase under 0.4 M NaCl (control group). The highest production of chlorophyll a (4.18 mg L⁻¹), allophycocyanin (4.08 mg L⁻¹), and phycoerythrin (1.70 mg L⁻¹) were achieved under 1.2 M NaCl conditions. Altered metabolic and lipidomic profiles were observed at different NaCl conditions; significantly increased relative yields of glucosylglycerol, one diacylglyceryltrimethylhomoserine, one monogalactosyldiacylglycerol, and four phosphatidylglycerol species were observed under 1.2 M NaCl conditions using gas chromatography–mass spectrometry and direct infusion–mass spectrometry analyses. In addition, it was revealed that the photosynthetic activity recovered under 1.2 M NaCl conditions in long-term culture. Hydrogen peroxide content significantly increased under 1.2 M NaCl conditions. It is believed that glutathione content also significantly increased under high salinity conditions to retain the normal functioning of Synechocystis 7338. These results indicate that high salinity conditions for Synechocystis 7338 culture could be used for the large-scale production of chlorophyll a, allophycocyanin, phycoerythrin, and other bioactive metabolites.

集胞藻 PCC 7338（以下简称为集胞藻7338）是具有产生光合色素的潜在海洋蓝细菌。在这项研究中，我们研究了各种浓度NaCl（0，0.4，0.8和1.2 M）对细胞生长，光合色素，和代谢物和完整的脂质种类谱的影响在集胞藻7338.的总体生长模式集胞藻7338相似在0、0.4和0.8 M NaCl条件下。在1.2 M NaCl下达到指数相后，细胞生长受到阻碍；然而，在0.4 M NaCl（对照组）下，在指数期后观察到相似的生长模式。叶绿素a的最高产量（4.18 mg L ^-1），藻蓝蛋白（4.08 mg L ^-1）和藻红蛋白（1.70 mg L ^-1）是在1.2 M NaCl条件下获得的。在不同的NaCl条件下观察到了代谢和脂质组学变化；在1.2 M NaCl条件下使用气相色谱-质谱和直接输注-质谱分析法观察到，葡萄糖基甘油，一种二酰基甘油三甲基高丝氨酸，一种单半乳糖基二酰基甘油和四种磷脂酰甘油的相对产率显着提高。另外，揭示了在长期培养中在1.2M NaCl条件下光合活性得以恢复。在1.2 M NaCl条件下，过氧化氢含量显着增加。人们认为，在高盐度条件下，谷胱甘肽的含量也显着增加，以保持集胞藻的正常功能。7338.这些结果表明，多裂藻7338培养物的高盐度条件可用于大规模生产叶绿素a，别藻蓝蛋白，藻红蛋白和其他生物活性代谢物。