Rhamnolipids are important biosurfactants for application in bioremediation, enhanced oil recovery, pharmaceutical, and detergent industry. In this study, rhamnolipids extracted from P. aeruginosa P6 were characterized to determine their potential fields of application. Thin-layer chromatographic analysis of the produced rhamnolipids indicated the production of two homologues: mono- and di-rhamnolipids, whose structures were verified by ¹H and ¹³C nuclear magnetic resonance spectroscopy. Additionally, high performance liquid chromatography-mass spectrometry identified seven different rhamnolipid congeners, of which a significantly high proportion was di-rhamnolipids reaching 80.16%. Rha-Rha-C10-C10 was confirmed as the principal compound of the rhamnolipid mixture (24.30%). The rhamnolipids were capable of lowering surface tension of water to 36 mN/m at a critical micelle concentration of 0.2 g/L, and exhibited a great emulsifying activity (E24 = 63%). In addition, they showed excellent stability at pH ranges 4–8, NaCl concentrations up to 9% (w/v) and temperatures ranging from 20 to 100 °C and even after autoclaving. These results suggest that rhamnolipids, produced by P. aeruginosa P6 using the cheap substrate glycerol, are propitious for biotechnology use in extreme and complex environments, like oil reservoirs and hydrocarbon contaminated soil. Moreover, P. aeruginosa P6 may be considered, in its wild type form, as a promising industrial producer of di-RLs, which have superior characteristics for potential applications and offer outstanding commercial benefits.

鼠李糖脂是重要的生物表面活性剂，应用于生物修复、提高采油率、制药和洗涤剂行业。在这项研究中，对从铜绿假单胞菌P6 中提取的鼠李糖脂进行了表征，以确定其潜在的应用领域。所产生的鼠李糖脂的薄层色谱分析表明产生了两种同系物：单鼠李糖脂和二鼠李糖脂，其结构通过¹ H 和¹³ C 核磁共振波谱验证。此外，高效液相色谱-质谱法还鉴定出7种不同的鼠李糖脂同系物，其中二鼠李糖脂的比例非常高，达到80.16%。 Rha-Rha-C10-C10被确认为鼠李糖脂混合物的主要化合物(24.30%)。鼠李糖脂在临界胶束浓度为0.2 g/L时能够将水的表面张力降低至36 mN/m，并表现出良好的乳化活性（E24 = 63%）。此外，它们在 pH 范围 4-8、氯化钠浓度高达 9% (w/v) 和温度范围 20 至 100 °C 甚至高压灭菌后都表现出出色的稳定性。这些结果表明，由铜绿假单胞菌P6 使用廉价底物甘油产生的鼠李糖脂有利于在极端和复杂环境（如油库和碳氢化合物污染的土壤）中使用生物技术。此外，野生型铜绿假单胞菌P6 可以被认为是一种有前途的 di-RL 工业生产者，其具有潜在应用的优异特性并提供显着的商业效益。