When PCR was first introduced for routine use in diagnostic laboratories in the 1980s, the technology opened the door to a new and dynamic future for detection of disease agents. However, the implementation of PCR technology in animal health laboratories far outpaced the development of documented guidelines and standards for use. In 2011, an effort was initiated within the American Association of Veterinary Laboratory Diagnosticians (AAVLD) membership to share PCR-based testing experiences more formally, as well as to document relevant laboratory science for the benefit of all animal health laboratories. The AAVLD “Laboratory Technology Committee” was formed during the annual 2011 AAVLD/USAHA meeting to provide a platform for laboratorians to exchange and discuss new testing technologies, including but not limited to molecular-based tests. The committee members devised a plan to combine the plethora of PCR-based testing experiences and best practices into general guidance documents to assist laboratories in establishing and performing PCR within their own unique laboratory settings. The committee formulated an overall goal of helping to provide consistent, high-quality molecular-based testing for the veterinary diagnostic laboratory community.

中文翻译：

---

重点关注 PCR 检测的开发、验证和使用的最佳实践

当 1980 年代首次将 PCR 引入诊断实验室的常规应用时，该技术为检测病原体打开了通往充满活力的新未来的大门。然而，PCR 技术在动物健康实验室的实施远远超过了书面指南和使用标准的发展。2011 年，美国兽医实验室诊断医师协会 (AAVLD) 成员开始努力更正式地分享基于 PCR 的检测经验，并记录相关实验室科学，以造福所有动物健康实验室。AAVLD“实验室技术委员会”成立于 2011 年 AAVLD/USAHA 年度会议期间，为实验室人员提供一个交流和讨论新测试技术的平台，包括但不限于基于分子的测试。委员会成员制定了一项计划，将大量基于 PCR 的测试经验和最佳实践结合到通用指导文件中，以帮助实验室在自己独特的实验室环境中建立和执行 PCR。该委员会制定了一个总体目标，即帮助为兽医诊断实验室社区提供一致的、高质量的基于分子的检测。