Abstract

In this study, we sequenced the complete mitochondrial genome of Eutrichosiphum pasaniae through Illumina platform. The circular mitogenome is 16,500 bp in length and composed of 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), 2 ribosomal RNA genes (rRNAs), a large control region and a special repeat region. The nucleotide composition of whole mitogenome is strongly AT-biased (85.5%). All PCGs start with ATN and end with TAA except for cox1 which terminates with an incomplete stop codon T. All tRNAs have a typical clover-leaf secondary structure except for trnS (AGN). The lengths of rrnL, rrnS and control region are 1276, 774 and 996 bp, respectively. The repeat region with a length of 909 bp is located between trnE and trnF and consists of 4.1 repeat units. The phylogenetic tree supports the sister relationship of Eutrichosiphum pasaniae and Greenidea psidii.

摘要

在这项研究中，我们通过Illumina平台测序了大叶紫苏线粒体的完整基因组。环状有丝分裂基因组的长度为16,500 bp，由13个蛋白质编码基因（PCG），22个转移RNA基因（tRNA），2个核糖体RNA基因（rRNA），较大的控制区和特殊的重复区组成。整个有丝分裂基因组的核苷酸组成强烈受到AT偏向（85.5％）。除cox1以不完整的终止密码子T终止外，所有PCG均以ATN开头并以TAA结尾。除trnS（AGN）外，所有tRNA均具有典型的三叶草叶二级结构。rrnL，rrnS的长度对照区和对照区分别为1276、774和996bp。长度为909 bp的重复区位于trnE和trnF之间，由4.1个重复单元组成。系统进化树支持帕氏真鳞草与绿蝇的姐妹关系。