The side effects and high cost of growth factors and drugs in bone tissue engineering have led to outstanding investigations of alternative osteoinductive supplements. Herein, the electrospun polycaprolactone (PCL) nanofibers were immobilized with carboxymethyl chitosan (CMC) and laponite nanoplatelets (LAP, 0.5‐3.5 wt%) to fabricate osteoinductive scaffolds for bone tissue engineering. The Fourier‐transform infrared)FTIR(and energy dispersive X‐ray (EDX) spectroscopy confirmed the chemical immobilization of CMC and LAP on the surface of PCL nanofibers. Water contact angle measurements exhibited significant improvement of surface hydrophilicity for immobilized scaffolds (<10°) comparing to the virgin PCL nanofibers (125°). The immobilization of CMC and LAP enhanced the attachment, proliferation, and osteodifferentiation of the seeded human bone marrow mesenchymal stem cells (hBM‐MSCs), as evidenced by increased calcium deposition, alkaline phosphatase (ALP) activity, and Osteonectin gene expression. Therefore, the fabricated scaffolds can serve as an appropriate substrate to support the proliferation and differentiation of stem cells to osteoplasts without any external osteoinductive stimulation.

中文翻译：

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羧甲基壳聚糖/膨润土在聚己内酯纳米纤维上作为骨诱导性骨支架的固定化

骨组织工程中生长因子和药物的副作用以及高昂的费用已导致对替代性骨诱导性补充剂的出色研究。在此，将静电纺丝的聚己内酯（PCL）纳米纤维与羧甲基壳聚糖（CMC）和合成锂皂石纳米片（LAP，0.5-3.5 wt％）固定在一起，以制备用于骨组织工程的骨诱导性支架。傅里叶变换红外（FTIR）光谱和能量色散X射线（EDX）光谱证实了CMC和LAP化学固定在PCL纳米纤维表面上。水接触角测量显示固定支架的表面亲水性显着提高（<10° ）与原始PCL纳米纤维（125°）相比。CMC和LAP的固定化增强了附着，增殖，碱性磷酸酶（ALP）活性和骨连接蛋白基因表达。因此，所制造的支架可以用作合适的底物以支持干细胞向骨质细胞的增殖和分化，而无需任何外部的骨诱导刺激。