Integrated analysis of phosphoproteome and ubiquitylome in epididymal sperm of buffalo (Bubalus bubalis),Molecular Reproduction and Development

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Integrated analysis of phosphoproteome and ubiquitylome in epididymal sperm of buffalo (Bubalus bubalis)
Molecular Reproduction and Development ( IF 2.7 ) Pub Date : 2020-11-02 , DOI: 10.1002/mrd.23432
Peng-Fei Zhang ₁ , Yu-Lin Huang ₂ , Qiang Fu ₁ , Weng-Tan He ₁ , Kai Xiao ₁ , Ming Zhang ₁

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In mammals, sperm need to mature in the epididymis to gain fertilization competency. However, the molecular mechanism underlying buffalo sperm maturation remains elusive. Exploring sperm physiology at the posttranslational modification (PTM) level could help to develop our understanding of these mechanisms. Protein phosphorylation and ubiquitination are major PTMs in the regulation of many biological processes. In the present study, to our knowledge, we report the first phosphoproteome and ubiquitylome of sperm collected from the caput, corpus, and cauda segments of the epididymis using liquid chromatography–mass spectrometry combined with affinity purification. In total, 647 phosphorylation sites in 294 proteins and 1063 ubiquitination sites in 446 proteins were characterized. Some of these proteins were associated with cellular developmental processes and energy metabolic pathways. Interestingly, 84 proteins were both phosphorylated and ubiquitinated, simultaneously. Some of these proteins were involved in, for example, spermatogenesis, reproduction, and spermatid development. Taken together, these data provide a theoretical basis for further functional analysis of phosphorylation and ubiquitination in epididymal sperm of buffalo and other mammals, and serve as an important resource for exploring the physiological mechanism underlying sperm maturation.

中文翻译：

水牛（Bubalus bubalis）附睾精子磷酸化蛋白质组和泛素组的综合分析

在哺乳动物中，精子需要在附睾中成熟才能获得受精能力。然而，水牛精子成熟的分子机制仍然难以捉摸。在翻译后修饰（PTM）水平上探索精子生理学有助于加深我们对这些机制的理解。蛋白质磷酸化和泛素化是许多生物过程调节中的主要 PTM。在本研究中，据我们所知，我们报告了使用液相色谱-质谱联用结合亲和纯化从附睾头、体和尾段收集的第一个精子磷酸化蛋白质组和泛素组。总共鉴定了 294 个蛋白质中的 647 个磷酸化位点和 446 个蛋白质中的 1063 个泛素化位点。其中一些蛋白质与细胞发育过程和能量代谢途径有关。有趣的是，84 种蛋白质同时被磷酸化和泛素化。其中一些蛋白质涉及精子发生、繁殖和精子细胞发育等。综上所述，这些数据为进一步分析水牛等哺乳动物附睾精子磷酸化和泛素化的功能提供了理论基础，并为探索精子成熟的生理机制提供了重要资源。

更新日期：2020-11-02

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