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Determination of cefixime in pure form, in pharmaceutical products and biological samples through fluorescence quenching of eosin Y
Luminescence ( IF 3.2 ) Pub Date : 2020-11-03 , DOI: 10.1002/bio.3971 Muhammad N Khan 1 , Irum 1 , Muhammad Mursaleen 1
Luminescence ( IF 3.2 ) Pub Date : 2020-11-03 , DOI: 10.1002/bio.3971 Muhammad N Khan 1 , Irum 1 , Muhammad Mursaleen 1
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A simple, economic and validated spectrofluorimetric method was developed to assay cefixime (CFX). The technique relies on the quenching effect of CFX on the fluorescence intensity of eosin Y in the presence of acetate buffer pH 3.4 to produce an ion‐pair complex that is measured at 549 nm using an excitation wavelength of 300 nm. Reaction‐influencing factors were carefully investigated and optimized. The fluorescence quenching value was linear to the CFX concentration in the range 0.2–40 μg/ml with a correlation coefficient of 0.992. The calculated limit of detection and limit of quantification were found to be 0.00242 and 0.0080 μg ml−1, respectively. The selectivity of the method was confirmed by studying the effects of excipients and no interference was distinguished. The developed method was used to determine CFX in marketable products and in biological samples. To validate the method, directives of the International Conference on Harmonization were applied and per cent recoveries obtained ranged from 95.30 to 102.50% for pharmaceutical products and from 97.00 to 103.00% for biological fluids.
中文翻译：
曙红Y荧光猝灭法测定药品和生物样品中的头孢克肟
开发了一种简单，经济且经过验证的分光荧光法来测定头孢克肟（CFX）。该技术依赖于在存在pH 3.4的乙酸盐缓冲液的情况下CFX对曙红Y荧光强度的淬灭作用，以产生离子对络合物，该络合物在300 nm的激发波长下于549 nm处测量。对反应影响因素进行了仔细研究和优化。荧光猝灭值在0.2–40μg/ ml范围内与CFX浓度呈线性关系，相关系数为0.992。发现计算出的检出限和定量限分别为0.00242和0.0080μgml -1， 分别。通过研究赋形剂的作用证实了该方法的选择性，并且没有发现干扰。所开发的方法用于测定可销售产品和生物样品中的CFX。为了验证该方法，应用了国际协调会议的指令，药物产品的回收率在95.30％至102.50％之间，生物流体的回收率在97.00％至103.00％之间。
更新日期：2020-11-03
中文翻译：
曙红Y荧光猝灭法测定药品和生物样品中的头孢克肟
开发了一种简单，经济且经过验证的分光荧光法来测定头孢克肟（CFX）。该技术依赖于在存在pH 3.4的乙酸盐缓冲液的情况下CFX对曙红Y荧光强度的淬灭作用，以产生离子对络合物，该络合物在300 nm的激发波长下于549 nm处测量。对反应影响因素进行了仔细研究和优化。荧光猝灭值在0.2–40μg/ ml范围内与CFX浓度呈线性关系，相关系数为0.992。发现计算出的检出限和定量限分别为0.00242和0.0080μgml -1， 分别。通过研究赋形剂的作用证实了该方法的选择性，并且没有发现干扰。所开发的方法用于测定可销售产品和生物样品中的CFX。为了验证该方法，应用了国际协调会议的指令，药物产品的回收率在95.30％至102.50％之间，生物流体的回收率在97.00％至103.00％之间。
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