Sandwich‐based surface‐enhanced Raman scattering probes for detection and quantification of malaria,Journal of Raman Spectroscopy

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Sandwich‐based surface‐enhanced Raman scattering probes for detection and quantification of malaria
Journal of Raman Spectroscopy ( IF 2.4 ) Pub Date : 2020-11-03 , DOI: 10.1002/jrs.6021
Nikiwe Mhlanga _{1,

2} , Thulaganyo Domfe _{1,

2,

3} , Amanda Skepu _{1,

2} , Thabang A. Ntho ₂

Affiliation

Malaria continues to be endemic in the middle‐ to low‐resourced regions of the world. One of the World Health Organization (WHO) global technical strategy goals towards 2030 is to lower the malaria infection by 90%. Attaining this ambitious goal entails early and accurate diagnosis of the parasite that will inform treatment measures to be taken. Herein, we report on the application of the surface‐enhanced Raman spectroscopy (SERS) platform to fabricate malaria bio‐sensing probes: lactate dehydrogenase (LDH) malaria antibodies (mAb) were immobilized on a SERS substrate and used to capture Plasmodium falciparum (pf) malaria antigen. A detection hybrid, Ag plasmonic metals labelled with a Raman reporter (SERS tag) and conjugated to a second LDH mAb, was hybridized on the captured antigen. The detection hybrid binds the antigen on a specific epitope, and the sandwich is interpreted using vibrational Raman spectroscopy that indirectly confirms the pfLDH malaria antigen via the Raman reporter label. The SERS probes showed specificity, sensitivity and rapidity in the detection of pfLDH. They are proposed for secondary confirmation of field‐based lateral flows and offer improved sensitivity and quantification capabilities.

中文翻译：

基于三明治的表面增强拉曼散射探针，用于检测和量化疟疾

疟疾在世界中到资源贫乏地区仍然很流行。世界卫生组织（WHO）到2030年的全球技术战略目标之一是将疟疾感染率降低90％。要实现这一宏伟目标，就需要对寄生虫进行早期而准确的诊断，这将为要采取的治疗措施提供依据。在此，我们报道了表面增强拉曼光谱（SERS）平台在制造疟疾生物传感探针上的应用：乳酸脱氢酶（LDH）疟疾抗体（mAb）被固定在SERS基质上并用于捕获恶性疟原虫（pf）疟疾抗原。将检测的杂合子（标记有拉曼报告基因（SERS标签）并与第二个LDH mAb缀合的Ag等离子体金属）与捕获的抗原杂交。检测杂合体将抗原结合在特定表位上，并使用振动拉曼光谱解释三明治，该振动拉曼光谱通过拉曼报道分子标签间接确认pfLDH疟疾抗原。SERS探针在检测pfLDH时显示出特异性，敏感性和快速性。建议将其用于基于现场的侧向流的二次确认，并提供更高的灵敏度和定量能力。

更新日期：2020-12-10

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