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Measurement of red blood cell size based on a lensless imaging system
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-11-02 , DOI: 10.1002/bab.2057 Jianwei Li 1 , Li Dai 1 , Ningmei Yu 1 , Zhengpeng Li 1 , Shuaijun Li 1
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-11-02 , DOI: 10.1002/bab.2057 Jianwei Li 1 , Li Dai 1 , Ningmei Yu 1 , Zhengpeng Li 1 , Shuaijun Li 1
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The variability in the size of red blood cells (RBCs) is an important additional diagnostic parameter for diseases, which has been established as a part of the complete blood count (CBC). The CBC can be performed using an automated flow cytometer, but it is too bulky and expensive for point-of-care testing. A miniaturized lensless imaging system is a competitive modality for a CBC, that is small and inexpensive. There are two challenges in developing a lensless imaging system for taking the CBC, which make the measurement of the RBC size very difficult: the diffraction effect and the low resolution. In this paper, the RBC radius measurement is replaced with a diffraction ring radius measurement. The diffraction ring radius is much larger than the RBC radius. This feature can improve the imaging resolution. Based on Fresnel diffraction, the relationship between the radius of RBCs and the diffraction fringes is analyzed. Finally, a complete measurement algorithm for determining the RBC size based on the lensless imaging system is given, which can be used to measure the variability in the size of RBCs. In our experiment, the maximum error is less than 6.74%.
中文翻译：
基于无透镜成像系统的红细胞大小测量
红细胞 (RBC) 大小的变异性是疾病的重要附加诊断参数，已被确定为全血细胞计数 (CBC) 的一部分。CBC 可以使用自动流式细胞仪进行，但对于即时检测来说过于庞大且昂贵。小型化无透镜成像系统是 CBC 的一种竞争方式，它体积小且价格低廉。开发用于获取 CBC 的无透镜成像系统存在两个挑战，这使得 RBC 尺寸的测量非常困难：衍射效应和低分辨率。在本文中，RBC 半径测量被衍射环半径测量所取代。衍射环半径远大于RBC半径。此功能可以提高成像分辨率。基于菲涅耳衍射，分析了红细胞半径与衍射条纹的关系。最后，给出了基于无透镜成像系统确定红细胞大小的完整测量算法，可用于测量红细胞大小的变异性。在我们的实验中，最大误差小于 6.74%。
更新日期：2020-11-02
中文翻译：
基于无透镜成像系统的红细胞大小测量
红细胞 (RBC) 大小的变异性是疾病的重要附加诊断参数，已被确定为全血细胞计数 (CBC) 的一部分。CBC 可以使用自动流式细胞仪进行，但对于即时检测来说过于庞大且昂贵。小型化无透镜成像系统是 CBC 的一种竞争方式，它体积小且价格低廉。开发用于获取 CBC 的无透镜成像系统存在两个挑战，这使得 RBC 尺寸的测量非常困难：衍射效应和低分辨率。在本文中，RBC 半径测量被衍射环半径测量所取代。衍射环半径远大于RBC半径。此功能可以提高成像分辨率。基于菲涅耳衍射，分析了红细胞半径与衍射条纹的关系。最后，给出了基于无透镜成像系统确定红细胞大小的完整测量算法，可用于测量红细胞大小的变异性。在我们的实验中，最大误差小于 6.74%。
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