Bile acid profiles in bile and feces of obese mice by a high-performance liquid chromatography–tandem mass spectrometry,Biotechnology and Applied Biochemistry

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Bile acid profiles in bile and feces of obese mice by a high-performance liquid chromatography–tandem mass spectrometry
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-11-02 , DOI: 10.1002/bab.2055
Junping Zheng _{1,

2} , Cheng Ye ₃ , Baifei Hu ₂ , Huabing Yang ₂ , Qunfeng Yao ₂ , Jun Ma ₂ , Yang Liu ₁ , Hongtao Liu _{1,

2}

Affiliation

Bile acids (BAs) play a pivotal role in manipulating the development of metabolic diseases. However, due to the compositional complexity and functional variation of BAs, it remains unclear about the changes in BA pool for individuals with obesity or metabolic syndrome. We established a high-performance liquid chromatography–mass spectrometer detection system for the simultaneous analysis of both unconjugated and conjugated BAs in the bile and feces of mice. Ten BAs were completely separated, identified, and quantified with low limit of detection (0.5 ng/mL) and inter/intraday precision (relative standard deviation < 12%). By using this method, these BAs in bile and feces of mice were quantified. The result showed that taurochenodeoxycholic acid, taurine-conjugated α-muricholic acids, and taurine-conjugated β-muricholic acids were the dominated BAs in bile, whereas deoxycholic acid and chenodeoxycholic acid predominated in feces. Further, most of the BA levels were significantly elevated in either bile or fecal samples of high-fat diet-fed mice as compared with those in normal chow diet-fed mice, indicating that excessive production of BAs was closely associated with the occurrence of lipid metabolism disorders. In summary, the present method is practicable for analysis of BAs in bile and fecal samples of patients with obesity.

中文翻译：

高效液相色谱-串联质谱法测定肥胖小鼠胆汁和粪便中的胆汁酸谱

胆汁酸 (BAs) 在控制代谢疾病的发展中发挥着关键作用。然而，由于 BA 的组成复杂性和功能变化，对于肥胖或代谢综合征患者的 BA 池变化仍不清楚。我们建立了一种高效液相色谱-质谱仪检测系统，用于同时分析小鼠胆汁和粪便中未结合和结合的 BA。十种 BA 被完全分离、鉴定和量化，检测限低 (0.5 ng/mL) 和日间/日内精密度 (相对标准偏差 < 12%)。通过使用这种方法，对小鼠胆汁和粪便中的这些 BAs 进行了量化。结果表明，牛磺鹅去氧胆酸、牛磺酸共轭α-鼠胆酸、和牛磺酸结合的β-鼠胆酸是胆汁中的主要BAs，而脱氧胆酸和鹅去氧胆酸在粪便中占优势。此外，与正常饮食喂养的小鼠相比，高脂饮食喂养小鼠的胆汁或粪便样本中的大多数 BA 水平显着升高，表明 BA 的过量产生与脂质的发生密切相关。代谢紊乱。总之，本方法可用于分析肥胖患者胆汁和粪便样本中的 BA。表明BAs的过量产生与脂质代谢紊乱的发生密切相关。总之，本方法可用于分析肥胖患者胆汁和粪便样本中的 BA。表明BAs的过量产生与脂质代谢紊乱的发生密切相关。总之，本方法可用于分析肥胖患者胆汁和粪便样本中的 BA。

更新日期：2020-11-02

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