Abstract A custom membrane extraction electrospray ionization mass spectrometry (MEESI-MS) method was developed to detect the representative bacteric metabolites and classify four kinds of bacteria including Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa. The chemical fingerprints of each bacterium were obtained using this MEESI-MS. Principal component analysis (PCA) was then applied to analyze the information from the obtained fingerprint spectra and classify the samples. The results showed that MEESI-MS was capable of rapidly obtaining metabolic fingerprints of blood samples and direct identifying the representative metabolites from each bacterium via employing both accurate mass measurements and structural information from tandem mass spectra. The overall analysis time was less than 65 min (including a culture time of about 60 min). This method was featured by the simplicity in sample pretreatment, ionization activation and data analysis and was promising in rapid identification of bacterial species in clinical bacteremia and septicemia.

中文翻译：

---

膜萃取电喷雾电离质谱法快速分析菌血症

摘要 开发了一种定制的膜萃取电喷雾电离质谱(MEESI-MS) 方法来检测具有代表性的细菌代谢物并对四种细菌进行分类，包括大肠杆菌、金黄色葡萄球菌、肺炎克雷伯菌和铜绿假单胞菌。使用该 MEESI-MS 获得每种细菌的化学指纹。然后应用主成分分析（PCA）来分析来自获得的指纹光谱的信息并对样本进行分类。结果表明，MEESI-MS 能够快速获得血液样本的代谢指纹，并通过采用准确的质量测量和串联质谱的结构信息直接识别每种细菌的代表性代谢物。总分析时间小于 65 分钟（包括约 60 分钟的培养时间）。该方法具有样品前处理、电离活化和数据分析简单等特点，有望用于临床菌血症和败血症中细菌种类的快速鉴定。